Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Aug;53(8):1913–1917. doi: 10.1128/aem.53.8.1913-1917.1987

Chemically Defined Medium for the Accumulation of Intracellular Malate Dehydrogenase by Streptomyces aureofaciens

Cecília Laluce 1,*, José Roberto Ernandes 1, Rubens Molinari 1
PMCID: PMC204024  PMID: 16347416

Abstract

A chemically defined medium was developed for the production of intracellular malate dehydrogenases by Streptomyces aureofaciens NRRL-B 1286. The composition of the medium (per liter) was as follows: 50 g of starch, 4 g of ammonium sulfate, 7.32 g of l-aspartic acid, 13.8 g of MgSO4 · 7H2O, 1.7 g of K2HPO4, 0.01 g of ZnSO4 · 7H2O, 0.01 g of FeSO4 · 7H2O, 0.01 g of MnSO4 · H2O, and 0.005 g of CoSO4 · 7H2O. The pH of the medium was adjusted to 6.7 to 7.0 after sterilization. The activity of the intracellular malate dehydrogenases of the crude cell extract was greatest after 40 h of mycelium growth in a rotary shaker at 30°C. The best temperature for the enzyme reactions was approximately 35°C for NAD+ activity at pH 9.7 and 40°C for NADP+ -linked enzyme at pH 9.0. The NAD+ activity required Mg2+, and both activities were sensitive to SH-group reagents. The NADP+ -dependent activity remained completely stable, and the NAD+ -dependent activity decreased to a very low residual level after 30 min at 60°C.

Full text

PDF
1913

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ITZHAKI R. F., GILL D. M. A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS. Anal Biochem. 1964 Dec;9:401–410. doi: 10.1016/0003-2697(64)90200-3. [DOI] [PubMed] [Google Scholar]
  2. Jechová V., Hostálek Z., Vanek Z. Regulation of biosynthesis of secondary metabolites. V. Decarboxylating malate dehydrogenase in Streptomyces aureofaciens. Folia Microbiol (Praha) 1969;14(2):128–134. doi: 10.1007/BF02892881. [DOI] [PubMed] [Google Scholar]
  3. Jechová V., Hostálek Z., Vanek Z. Regulation of biosynthesis of secondary metabolites. XVII. Purification and properties of malate dehydrogenase (decarboxylating) in Streptomyces aureofaciens. Folia Microbiol (Praha) 1975;20(2):137–141. doi: 10.1007/BF02876770. [DOI] [PubMed] [Google Scholar]
  4. KORKES S., DEL CAMPILLO A., OCHOA S. Biosynthesis of dicarboxylic acids by carbon dioxide fixation. IV. Isolation and properties of an adaptive "malic" enzyme from Lactobacillus arabinosus. J Biol Chem. 1950 Dec;187(2):891–905. [PubMed] [Google Scholar]
  5. Laluce C., Molinari R. Selection of a chemically defined medium for submerged cultivation of Streptomyces aureofaciens with high extracellular caseinolytic activity. Biotechnol Bioeng. 1977 Dec;19(12):1863–1884. doi: 10.1002/bit.260191210. [DOI] [PubMed] [Google Scholar]
  6. Macrae A. R. Isolation and properties of a 'malic' enzyme from cauliflower bud mitochondria. Biochem J. 1971 May;122(4):495–501. doi: 10.1042/bj1220495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mandella R. D., Sauer L. A. The mitochondrial malic enzymes. I. Submitochondrial localization and purification and properties of the NAD(P)+-dependent enzyme from adrenal cortex. J Biol Chem. 1975 Aug 10;250(15):5877–5884. [PubMed] [Google Scholar]
  8. Murai T., Tokushige M., Nagai J., Katsuki H. Physiological functions of NAD- and NADP-linked malic enzymes in Escherichia coli. Biochem Biophys Res Commun. 1971 May 21;43(4):875–881. doi: 10.1016/0006-291x(71)90698-x. [DOI] [PubMed] [Google Scholar]
  9. Müller J., Klein C. Stability of dehydrogenases. III. Malate dehydrogenases. Biochim Biophys Acta. 1982 Sep 22;707(1):133–141. doi: 10.1016/0167-4838(82)90406-x. [DOI] [PubMed] [Google Scholar]
  10. ROBINSON J. C., KEAY L., MOLINARI R., SIZER I. W. L-alpha-Hydroxy acid oxidases of hog renal cortex. J Biol Chem. 1962 Jun;237:2001–2010. [PubMed] [Google Scholar]
  11. Shore J. D., Chakrabarti S. K. Subunit dissociation of mitochondrial malate dehydrogenase. Biochemistry. 1976 Feb 24;15(4):875–879. doi: 10.1021/bi00649a023. [DOI] [PubMed] [Google Scholar]
  12. Tinterová M., Hostálek Z., Vanek Z. Regulation of biosynthesis of secondary metabolities. VI. Characteristics of the isoenzymes of malate dehydrogenase in Streptomyces aureofaciens. Folia Microbiol (Praha) 1969;14(2):135–140. doi: 10.1007/BF02892882. [DOI] [PubMed] [Google Scholar]
  13. Zink M. W., Shaw D. A. Regulation of "malic" isozymes and malic dehydrogenases in Neurospora crassa. Can J Microbiol. 1968 Aug;14(8):907–912. doi: 10.1139/m68-152. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES