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Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Aug;53(8):1959–1961. doi: 10.1128/aem.53.8.1959-1961.1987

Transposon Mutants of Bradyrhizobium japonicum Altered in Attachment to Host Roots

Stephen J Vesper 1,*, Nasir S A Malik 1, Wolfgang D Bauer 1
PMCID: PMC204034  PMID: 16347421

Abstract

Transposon mutants of Bradyrhizobium japonicum 110 ARS were produced and screened for changes in attachment ability. Mutant CFK4 produced twice as many piliated cells, attached in 2.5-fold-higher numbers to soybean root segments, and colonized roots in about 2-fold-higher numbers than did the parental strain, 110 ARS. Mutants CFK35 and CFK38 were reduced in their attachment about 2-fold and 3.5-fold, respectively. This corresponded to reductions in piliated cells in their populations, reduced reaction with anti-pilus antiserum, and reduced hydrophobic attachment. Mutants CFK4 and CFK38 nodulated soybeans at about the same level as the parent strain, but CFK35 induced only pseudonodules. Two-dimensional gel analyses of the proteins from the mutants showed relatively few changes in proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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