Abstract
Transposon mutants of Bradyrhizobium japonicum 110 ARS were produced and screened for changes in attachment ability. Mutant CFK4 produced twice as many piliated cells, attached in 2.5-fold-higher numbers to soybean root segments, and colonized roots in about 2-fold-higher numbers than did the parental strain, 110 ARS. Mutants CFK35 and CFK38 were reduced in their attachment about 2-fold and 3.5-fold, respectively. This corresponded to reductions in piliated cells in their populations, reduced reaction with anti-pilus antiserum, and reduced hydrophobic attachment. Mutants CFK4 and CFK38 nodulated soybeans at about the same level as the parent strain, but CFK35 induced only pseudonodules. Two-dimensional gel analyses of the proteins from the mutants showed relatively few changes in proteins.
Full text
PDF![1959](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df37/204034/a29e4626e76f/aem00125-0247.png)
![1960](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df37/204034/42661f1a256d/aem00125-0248.png)
![1961](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df37/204034/836b5701c180/aem00125-0249.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bhuvaneswari T. V., Mills K. K., Crist D. K., Evans W. R., Bauer W. D. Effects of culture age on symbiotic infectivity of Rhizobium japonicum. J Bacteriol. 1983 Jan;153(1):443–451. doi: 10.1128/jb.153.1.443-451.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones G. W., Isaacson R. E. Proteinaceous bacterial adhesins and their receptors. Crit Rev Microbiol. 1983;10(3):229–260. doi: 10.3109/10408418209113564. [DOI] [PubMed] [Google Scholar]
- Kehoe M., Sellwood R., Shipley P., Dougan G. Genetic analysis of K88-mediated adhesion of enterotoxigenic Escherichia coli. Nature. 1981 May 14;291(5811):122–126. doi: 10.1038/291122a0. [DOI] [PubMed] [Google Scholar]
- Korhonen T. K., Tarkka E., Ranta H., Haahtela K. Type 3 fimbriae of Klebsiella sp.: molecular characterization and role in bacterial adhesion to plant roots. J Bacteriol. 1983 Aug;155(2):860–865. doi: 10.1128/jb.155.2.860-865.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maurer L., Orndorff P. E. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol. 1987 Feb;169(2):640–645. doi: 10.1128/jb.169.2.640-645.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orndorff P. E., Falkow S. Identification and characterization of a gene product that regulates type 1 piliation in Escherichia coli. J Bacteriol. 1984 Oct;160(1):61–66. doi: 10.1128/jb.160.1.61-66.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orndorff P. E., Falkow S. Organization and expression of genes responsible for type 1 piliation in Escherichia coli. J Bacteriol. 1984 Aug;159(2):736–744. doi: 10.1128/jb.159.2.736-744.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vesper S. J., Bauer W. D. Role of Pili (Fimbriae) in Attachment of Bradyrhizobium japonicum to Soybean Roots. Appl Environ Microbiol. 1986 Jul;52(1):134–141. doi: 10.1128/aem.52.1.134-141.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]