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. 1987 Aug;53(8):1969–1972. doi: 10.1128/aem.53.8.1969-1972.1987

Inadequacy of the Eucaryote Inhibitor Cycloheximide in Studies of Protozoan Grazing on Bacteria at the Freshwater-Sediment Interface

Sarah C Tremaine 1, Aaron L Mills 1,*
PMCID: PMC204037  PMID: 16347423

Abstract

Four guilds from a lake sediment-water interface microbial community were isolated and tested for sensitivity to cycloheximide (0.1 to 200 mg liter−1). Field experiments were conducted to compare the inhibition, dilution, and filtration methods for determining grazing rates. Cycloheximide inhibited anaerobic bacteria at 50 mg liter−1, and inhibition of bacterial growth was observed in the grazing experiments. The results show that the assumption of selective inhibition of heterotrophic eucaryotes was violated and preclude the use of cycloheximide in grazing experiments.

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1969

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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