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Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Aug;53(8):1977–1979. doi: 10.1128/aem.53.8.1977-1979.1987

Determining [3H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

P Servais 1,*, J Martinez 1, G Billen 1, J Vives-Rego 1
PMCID: PMC204039  PMID: 16347424

Abstract

Determination of [3H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method, the fraction of thymidine incorporated into DNA ranged from 10 to 83%.

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1977

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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