Abstract
The wall of intact Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis was investigated. Smaller cysts were identified within the soma of neurones. With larger cysts, the contained cystozoites were shown by ultrastructural examination to be surrounded by a layer of microtubules; immunohistochemical staining revealed that this layer contained neurofilament protein. Interior to this layer was a much convoluted parasitophorus vacuole membrane; exterior was the host cell membrane. In most cases, synaptic plates were noted on the outer plasma membrane. In no instance were tissue cysts observed either within neuroglial cells or in the absence of host cells. These observations are discussed in relation to the pathogenesis of congenital toxoplasmosis in the brain.
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- Bray D., Gilbert D. Cytoskeletal elements in neurons. Annu Rev Neurosci. 1981;4:505–523. doi: 10.1146/annurev.ne.04.030181.002445. [DOI] [PubMed] [Google Scholar]
- Desmonts G., Remington J. S. Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity. J Clin Microbiol. 1980 Jun;11(6):562–568. doi: 10.1128/jcm.11.6.562-568.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferguson D. J., Hutchison W. M. An ultrastructural study of the early development and tissue cyst formation of Toxoplasma gondii in the brains of mice. Parasitol Res. 1987;73(6):483–491. doi: 10.1007/BF00535321. [DOI] [PubMed] [Google Scholar]
- Ferguson D. J., Hutchison W. M. The host-parasite relationship of Toxoplasma gondii in the brains of chronically infected mice. Virchows Arch A Pathol Anat Histopathol. 1987;411(1):39–43. doi: 10.1007/BF00734512. [DOI] [PubMed] [Google Scholar]
- Frenkel J. K., Escajadillo A. Cyst rupture as a pathogenic mechanism of toxoplasmic encephalitis. Am J Trop Med Hyg. 1987 May;36(3):517–522. doi: 10.4269/ajtmh.1987.36.517. [DOI] [PubMed] [Google Scholar]
- Ghatak N. R., Zimmerman H. M. Fine structure of Toxoplasma in the human brain. Arch Pathol. 1973 Apr;95(4):276–283. [PubMed] [Google Scholar]
- Graham D. I., Hay J., Hutchison W. M., Siim J. C. Encephalitis in mice with congenital ocular toxoplasmosis. J Pathol. 1984 Apr;142(4):265–277. doi: 10.1002/path.1711420405. [DOI] [PubMed] [Google Scholar]
- Hay J., Aitken P. P., Arnott M. A. The influence of congenital Toxoplasma infection on the spontaneous running activity of mice. Z Parasitenkd. 1985;71(4):459–462. doi: 10.1007/BF00928348. [DOI] [PubMed] [Google Scholar]
- Hay J., Aitken P. P., Graham D. I. Toxoplasma infection and response to novelty in mice. Z Parasitenkd. 1984;70(5):575–588. doi: 10.1007/BF00926588. [DOI] [PubMed] [Google Scholar]
- Hay J., Aitken P. P., Hutchison W. M., Graham D. I. The effect of congenital and adult-acquired Toxoplasma infections on the motor performance of mice. Ann Trop Med Parasitol. 1983 Jun;77(3):261–277. doi: 10.1080/00034983.1983.11811707. [DOI] [PubMed] [Google Scholar]
- Hay J., Graham D. I., Dutton G. N., Logan S. The immunocytochemical demonstration of Toxoplasma antigen in the brains of congenitally infected mice. Z Parasitenkd. 1986;72(5):609–615. doi: 10.1007/BF00925482. [DOI] [PubMed] [Google Scholar]
- Hay J., Graham D. I., Lee W. R., Dutton G. N., Hutchison W. M. Congenital neuro-ophthalmic toxoplasmosis in the mouse. Ann Trop Med Parasitol. 1987 Feb;81(1):25–28. doi: 10.1080/00034983.1987.11812085. [DOI] [PubMed] [Google Scholar]
- Hay J., Hutchison W. M., Lee W. R., Siim J. C. Cataract in mice congenitally infected with Toxoplasma gondii. Ann Trop Med Parasitol. 1981 Aug;75(4):455–457. doi: 10.1080/00034983.1981.11687464. [DOI] [PubMed] [Google Scholar]
- Hutchison W. M. Strain of mouse and Toxoplasma used in the mouse model of congenital toxoplasmosis at Strathclyde University. Ann Trop Med Parasitol. 1986 Apr;80(2):253–255. doi: 10.1080/00034983.1986.11812010. [DOI] [PubMed] [Google Scholar]
- Jones T. C., Bienz K. A., Erb P. In vitro cultivation of Toxoplasma gondii cysts in astrocytes in the presence of gamma interferon. Infect Immun. 1986 Jan;51(1):147–156. doi: 10.1128/iai.51.1.147-156.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luft B. J., Conley F., Remington J. S., Laverdiere M., Wagner K. F., Levine J. F., Craven P. C., Strandberg D. A., File T. M., Rice N. Outbreak of central-nervous-system toxoplasmosis in western Europe and North America. Lancet. 1983 Apr 9;1(8328):781–784. doi: 10.1016/s0140-6736(83)91847-0. [DOI] [PubMed] [Google Scholar]
- Uga S., Okada S., Matsumura T. Proliferation of Toxoplasma gondii and its cyst-formation in mouse brains. Kobe J Med Sci. 1980 Dec;26(4):253–267. [PubMed] [Google Scholar]
- WANKO T., JACOBS L., GAVIN M. A. Electron microscope study of Toxoplasma cysts in mouse brain. J Protozool. 1962 May;9:235–242. doi: 10.1111/j.1550-7408.1962.tb02611.x. [DOI] [PubMed] [Google Scholar]
- Wilson C. B., Remington J. S., Stagno S., Reynolds D. W. Development of adverse sequelae in children born with subclinical congenital Toxoplasma infection. Pediatrics. 1980 Nov;66(5):767–774. [PubMed] [Google Scholar]
- Wong B., Gold J. W., Brown A. E., Lange M., Fried R., Grieco M., Mildvan D., Giron J., Tapper M. L., Lerner C. W. Central-nervous-system toxoplasmosis in homosexual men and parenteral drug abusers. Ann Intern Med. 1984 Jan;100(1):36–42. doi: 10.7326/0003-4819-100-1-36. [DOI] [PubMed] [Google Scholar]
- van der Zypen E. Licht- und Elektronenmikroskopische Studien zur Frage der Entwicklung von Toxoplasma-Cysten im Gehirn der weissen Maus. Z Parasitenkd. 1966;28(1):31–44. [PubMed] [Google Scholar]