Abstract
The effect of various media constituents on the production and measurement of luminol-dependent chemiluminescence by neutrophils stimulated by opsonized zymosan was studied. Glucose, calcium ions and magnesium ions were found to be necessary for optimal chemiluminescence although high concentrations of glucose had a detrimental effect. For our luminol-dependent system, a pH of 8.5 give maximal readings. The use of simple balanced salt solutions gave higher chemiluminescence responses than did more complex media with added protein, amino acids, and vitamins. However, the latter were important in the maintenance of optimal cell function.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andersen B. R., Amirault H. J. Important variables in granulocyte chemiluminescence. Proc Soc Exp Biol Med. 1979 Oct;162(1):139–145. doi: 10.3181/00379727-162-40633. [DOI] [PubMed] [Google Scholar]
- Bostick D. T., Hercules D. M. Quantitative determination of blood glucose using enzyme induced chemiluminescence of luminol. Anal Chem. 1975 Mar;47(3):447–452. doi: 10.1021/ac60353a039. [DOI] [PubMed] [Google Scholar]
- Easmon C. S., Cole P. J., Williams A. J., Hastings M. The measurement of opsonic and phagocytic function by Luminol-dependent chemiluminescence. Immunology. 1980 Sep;41(1):67–74. [PMC free article] [PubMed] [Google Scholar]
- Nelson R. D., Herron M. J., Schmidtke J. R., Simmons R. L. Chemiluminescence response of human leukocytes: influence of medium components on light production. Infect Immun. 1977 Sep;17(3):513–520. doi: 10.1128/iai.17.3.513-520.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens P., Winston D. J., Van Dyke K. In vitro evaluation of opsonic and cellular granulocyte function by luminol-dependent chemiluminescence: utility in patients with severe neutropenia and cellular deficiency states. Infect Immun. 1978 Oct;22(1):41–51. doi: 10.1128/iai.22.1.41-51.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens P., Young L. S. Quantitative granulocyte chemiluminescence in the rapid detection of impaired opsonization of Escherichia coli. Infect Immun. 1977 Jun;16(3):796–804. doi: 10.1128/iai.16.3.796-804.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takasugi M. An improved fluorochromatic cytotoxic test. Transplantation. 1971 Aug;12(2):148–151. doi: 10.1097/00007890-197108000-00010. [DOI] [PubMed] [Google Scholar]
- Van Oss C. J. Influence of glucose levels on the in vitro phagocytosis of bacteria by human neutrophils. Infect Immun. 1971 Jul;4(1):54–59. doi: 10.1128/iai.4.1.54-59.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]