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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1983 Apr;64(2):198–206.

The effect of complement-depletion or mannosyl receptor blockade on the in vivo clearance of antigen during a primary immune response in rabbits.

G D Harkiss, D L Brown
PMCID: PMC2040692  PMID: 6303380

Abstract

The effect of complement-depletion mediated by cobra venom factor or mannosyl receptor blockade induced by ovalbumin injections on the blood clearance of in vivo-generated soluble immune complexes were analysed in 2 variants of a primary immune elimination model system in rabbits. In the first variant, animals were immunized with particulate antigen to elicit high titre IgM antibodies, and received radiolabelled antigen on the first day of immunization. In the second variant, radiolabelled antigen was introduced on Day 8 of the immunization schedule. The results showed that (a) immune clearance of antigen (Days 6-8) was not inhibited by prior decomplementation of the animals with cobra venom factor, and (b) injection of ovalbumin just prior to immune clearance did not significantly alter the clearance kinetics of the resulting immune complexes. Thus, the results indicate that clearance of soluble immune complexes generated during a primary immune response is not mediated via C3b-C3b receptor interaction or via mannosyl-mannosyl receptor interaction in the reticuloendothelial system, but proceeds through other mechanisms perhaps involving Fc receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colten H. R. Biosynthesis of complement. Adv Immunol. 1976;22:67–118. doi: 10.1016/s0065-2776(08)60548-9. [DOI] [PubMed] [Google Scholar]
  2. Day J. F., Thornburg R. W., Thorpe S. R., Baynes J. W. Carbohydrate-mediated clearance of antibody . antigen complexes from the circulation. The role of high mannose oligosaccharides in the hepatic uptake of IgM . antigen complexes. J Biol Chem. 1980 Mar 25;255(6):2360–2365. [PubMed] [Google Scholar]
  3. Finbloom D. S., Plotz P. H. Studies of reticuloendothelial function in the mouse with model immune complexes. I. Serum clearance and tissue uptake in normal C3H mice. J Immunol. 1979 Oct;123(4):1594–1599. [PubMed] [Google Scholar]
  4. Harkiss G. D., Brown D. L. Clearance kinetics of soluble pre-formed immune complexes containing IgM antibodies in normal and decomplemented rabbits. Immunology. 1981 Feb;42(2):297–306. [PMC free article] [PubMed] [Google Scholar]
  5. Landen B., Strunkheide K., Dierich M. P. C3-mediated cytoadherence. II. Dependence of cell attachment on similar topographic distribution of the receptors (for C3) and the ligands (C3/C3b). J Immunol. 1978 Dec;121(6):2539–2541. [PubMed] [Google Scholar]
  6. Mannik M., Arend M. P., Hall A. P., Gilliland B. C. Studies on antigen-antibody complexes. I. Elimination of soluble complexes from rabbit circulation. J Exp Med. 1971 Apr 1;133(4):713–739. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mantovani B. Phagocytosis of immune complexes mediated by IgM and C3 receptors by macrophages from mice treated with glycogen. J Immunol. 1981 Jan;126(1):127–130. [PubMed] [Google Scholar]
  8. Miller G. W., Nussenzweig V. A new complement function: solubilization of antigen-antibody aggregates. Proc Natl Acad Sci U S A. 1975 Feb;72(2):418–422. doi: 10.1073/pnas.72.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Plotz P. H., Kimberly R. P., Guyer R. L., Segal D. M. Stable model immune complexes produced by bivalent affinity labeling haptens: in-vivo survival. Mol Immunol. 1979 Sep;16(9):721–729. doi: 10.1016/0161-5890(79)90013-0. [DOI] [PubMed] [Google Scholar]
  10. Rifai A., Finbloom D. S., Magilavy D. B., Plotz P. H. Modulation of the circulation and hepatic uptake of immune complexes by carbohydrate recognition systems. J Immunol. 1982 May;128(5):2269–2275. [PubMed] [Google Scholar]
  11. Schifferli J. A., Bartolotti S. R., Peters D. K. Inhibition of immune precipitation by complement. Clin Exp Immunol. 1980 Nov;42(2):387–394. [PMC free article] [PubMed] [Google Scholar]
  12. Schreiber A. D., Frank M. M. Role of antibody and complement in the immune clearance and destruction of erythrocytes. I. In vivo effects of IgG and IgM complement-fixing sites. J Clin Invest. 1972 Mar;51(3):575–582. doi: 10.1172/JCI106846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TORRIGIANI G., ROITT I. M. THE ENHANCEMENT OF 19S ANTIBODY PRODUCTION BY PARTICULATE ANTIGEN. J Exp Med. 1965 Jul 1;122:181–193. doi: 10.1084/jem.122.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WEIGLE W. O. Elimination of antigen-antibody complexes from sera of rabbits. J Immunol. 1958 Sep;81(3):204–213. [PubMed] [Google Scholar]

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