Skip to main content
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1982 Dec;63(6):644–650.

The influence of catecholamines and prostaglandins on calcium efflux and filtrability of thermally damaged erythrocytes: an in vitro study.

S Baar
PMCID: PMC2040698  PMID: 6960925

Abstract

Whole blood was heated in vitro and incubated with any of the following: epinephrine, norepinephrine, prostaglandin E1, prostaglandin E2 or prostaglandin F2 alpha. The treatment was assessed by whole-blood filtration and extrusion of a Ca2+ load from Ca2+ loaded red cells. Epinephrine did not affect the parameters measured. Norepinephrine increased cell filtration times and delayed Ca2+ extrusion. Prostaglandin E1 acted in an opposite manner. prostaglandin E2 and F2 alpha both decreased red-cell deformability and Ca2+ extrusion above the effect of heat alone Prostaglandin E2 showed the most pronounced effect. Possible implications of the findings for microcirculation in burned patients are discussed.

Full text

PDF
644

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen J. E., Rasmussen H. Human red blood cells: prostaglandin E2, epinephrine, and isoproterenol alter deformability. Science. 1971 Oct 29;174(4008):512–514. doi: 10.1126/science.174.4008.512. [DOI] [PubMed] [Google Scholar]
  2. Arturson G., Jonsson C. E. Effects of indomethacin on the transcapillary leakage of macromolecules and the efflux of prostaglandins in the paw lymph following experimental scalding injury. Ups J Med Sci. 1973 Jul;78(3):181–188. doi: 10.3109/03009737309178625. [DOI] [PubMed] [Google Scholar]
  3. Baar S. A convenient and reproducible filtration technique for the determination of erythrocyte flexibility. Br J Haematol. 1976 Sep;34(1):69–78. doi: 10.1111/j.1365-2141.1976.tb00175.x. [DOI] [PubMed] [Google Scholar]
  4. Baar S., Arrowsmith D. J. Thermal damage to red cells. J Clin Pathol. 1970 Oct;23(7):572–576. doi: 10.1136/jcp.23.7.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baar S. Mechanisms of delayed red cell destruction after thermal injury. An experimental in vitro sem study. Br J Exp Pathol. 1974 Apr;55(2):187–193. [PMC free article] [PubMed] [Google Scholar]
  6. Baar S. Water movement in red cells from burned patients. Its relationship to sodium retention and red cell filtrability. Clin Chim Acta. 1979 Jun 1;94(2):181–189. doi: 10.1016/0009-8981(79)90011-1. [DOI] [PubMed] [Google Scholar]
  7. Curreri P. W., Wilmore D. W., Mason A. D., Jr, Newsome T. W., Asch M. J., Pruitt B. A., Jr Intracellular cation alterations following major trauma: effect of supranormal caloric intake. J Trauma. 1971 May;11(5):390–396. doi: 10.1097/00005373-197105000-00003. [DOI] [PubMed] [Google Scholar]
  8. Dunn M. J. Red blood cell calcium and magnesium: effects upon sodium and potassium transport and cellular morphology. Biochim Biophys Acta. 1974 May 30;352(1):97–116. doi: 10.1016/0005-2736(74)90182-5. [DOI] [PubMed] [Google Scholar]
  9. Gardner J. D., Ginzler E. R. Sodium transport in human erythrocytes--absence of an effect of prostaglandin E1. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1063–1067. doi: 10.1016/0006-291x(71)90012-x. [DOI] [PubMed] [Google Scholar]
  10. Heggers J. P., Loy G. L., Robson M. C., Del Beccaro E. J. Histological demonstration of prostaglandins and thromboxanes in burned tissue. J Surg Res. 1980 Feb;28(2):110–117. doi: 10.1016/0022-4804(80)90153-5. [DOI] [PubMed] [Google Scholar]
  11. Johnston C. C., Dowers S. L., Urbanski R. J. Examination of the filterability of oxygenated erythrocytes (containing normal, trait or sickle cell disease type hemoglobins) in the presence of L-epinephrine, D,L-isoproterenol or prostaglandins (PG) A1, A2, E1, E2, F1alpha or F2alpha. Prostaglandins. 1977 Feb;13(2):281–309. doi: 10.1016/0090-6980(77)90009-0. [DOI] [PubMed] [Google Scholar]
  12. Kirkpatrick F. H., Hillman D. G., La Celle P. L. A23187 and red cells: changes in deformability, K+, Mg-2+, Ca-2+ and ATP. Experientia. 1975 Jun 15;31(6):653–654. doi: 10.1007/BF01944610. [DOI] [PubMed] [Google Scholar]
  13. Kury P. G., Ramwell P. W., McConnell H. M. The effect of prostaglandins E1 and E2 on the human erythrocyte as monitored by spin labels. Biochem Biophys Res Commun. 1974 Jan 23;56(2):478–483. doi: 10.1016/0006-291x(74)90867-5. [DOI] [PubMed] [Google Scholar]
  14. Lorand L., Siefring G. E., Jr, Lowe-Krentz L. Enzymatic basis of membrane stiffening in human erythroyctes. Semin Hematol. 1979 Jan;16(1):65–74. [PubMed] [Google Scholar]
  15. Sarkadi B., Szász I., Gárdos G. The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies. J Membr Biol. 1976 May;26(4):357–370. doi: 10.1007/BF01868883. [DOI] [PubMed] [Google Scholar]
  16. Schatzmann H. J. ATP-dependent Ca++-extrusion from human red cells. Experientia. 1966 Jun 15;22(6):364–365. doi: 10.1007/BF01901136. [DOI] [PubMed] [Google Scholar]
  17. Weed R. I., LaCelle P. L., Merrill E. W. Metabolic dependence of red cell deformability. J Clin Invest. 1969 May;48(5):795–809. doi: 10.1172/JCI106038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wilmore D. W., Long J. M., Mason A. D., Jr, Skreen R. W., Pruitt B. A., Jr Catecholamines: mediator of the hypermetabolic response to thermal injury. Ann Surg. 1974 Oct;180(4):653–669. doi: 10.1097/00000658-197410000-00031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ziegler M. G., Lake C. R., Kopin I. J. Plasma noradrenaline increases with age. Nature. 1976 May 27;261(5558):333–335. doi: 10.1038/261333a0. [DOI] [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES