Abstract
Spore morphology is currently used to identify species of vesicular-arbuscular mycorrhizal fungi. We report the first use of a highly specific immunological method for identification of a vesicular-arbuscular mycorrhizal fungus. Two monoclonal antibodies were produced against Glomus occultum. Monoclonal antibodies reacted strongly with both spores and hyphae in an indirect enzyme-linked immunosorbent assay. All other mycorrhizal (29 species) and nonmycorrhizal (5 species) fungi tested were nonreactive with the monoclonal antibodies. A single spore of G. occultum was detectable in the presence of high numbers of spores of other vesicular-arbuscular mycorrhizal fungi. Variation in the reaction of G. occultum isolates from West Virginia, Florida, and Colombia suggests that monoclonal antibodies may differentiate strains.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Wright S. F., Foster J. G., Bennett O. L. Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii. Appl Environ Microbiol. 1986 Jul;52(1):119–123. doi: 10.1128/aem.52.1.119-123.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]