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. 1987 Sep;53(9):2256–2259. doi: 10.1128/aem.53.9.2256-2259.1987

Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

A R Datta 1, B A Wentz 1, W E Hill 1
PMCID: PMC204091  PMID: 2823710

Abstract

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Audurier A., Pardon P., Marly J., Lantier F. Experimental infection of mice with Listeria monocytogenes and L. innocua. Ann Microbiol (Paris) 1980 Jul-Aug;131B(1):47–57. [PubMed] [Google Scholar]
  2. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  3. Gaillard J. L., Berche P., Sansonetti P. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun. 1986 Apr;52(1):50–55. doi: 10.1128/iai.52.1.50-55.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Groves R. D., Welshimer H. J. Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions. J Clin Microbiol. 1977 Jun;5(6):559–563. doi: 10.1128/jcm.5.6.559-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayes P. S., Feeley J. C., Graves L. M., Ajello G. W., Fleming D. W. Isolation of Listeria monocytogenes from raw milk. Appl Environ Microbiol. 1986 Feb;51(2):438–440. doi: 10.1128/aem.51.2.438-440.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heath L. S., Sloan G. L., Heath H. E. A simple and generally applicable procedure for releasing DNA from bacterial cells. Appl Environ Microbiol. 1986 May;51(5):1138–1140. doi: 10.1128/aem.51.5.1138-1140.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JENKINS E. M., NJOKU-OBI A. N., ADAMS E. W. PURIFICATION OF THE SOLUBLE HEMOLYSINS OF LISTERIA MONOCYTOGENES. J Bacteriol. 1964 Aug;88:418–424. doi: 10.1128/jb.88.2.418-424.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kathariou S., Metz P., Hof H., Goebel W. Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes. J Bacteriol. 1987 Mar;169(3):1291–1297. doi: 10.1128/jb.169.3.1291-1297.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maas R. An improved colony hybridization method with significantly increased sensitivity for detection of single genes. Plasmid. 1983 Nov;10(3):296–298. doi: 10.1016/0147-619x(83)90045-8. [DOI] [PubMed] [Google Scholar]
  11. Parrisius J., Bhakdi S., Roth M., Tranum-Jensen J., Goebel W., Seeliger H. P. Production of listeriolysin by beta-hemolytic strains of Listeria monocytogenes. Infect Immun. 1986 Jan;51(1):314–319. doi: 10.1128/iai.51.1.314-319.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Siddique I. H., Lin I. F., Chung R. A. Purification and characterization of hemolysin produced by Listeria monocytogenes. Am J Vet Res. 1974 Feb;35(2):289–286. [PubMed] [Google Scholar]
  13. Skalka B., Smola J., Elischerová K. Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes strains. J Clin Microbiol. 1982 Mar;15(3):503–507. doi: 10.1128/jcm.15.3.503-507.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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