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. 1987 Sep;53(9):2260–2261. doi: 10.1128/aem.53.9.2260-2261.1987

13C Nuclear Magnetic Resonance Studies of Propionate Catabolism in Methanogenic Cocultures †

J E Robbins 1
PMCID: PMC204092  PMID: 16347447

Abstract

Propionate catabolism was monitored in anaerobic cocultures of propionate-degrading and methanogenic bacteria. Metabolism was monitored by use of 13C-enriched propionate and succinate. The intermediates identified indicated that the methylmalonyl coenzyme A pathway was used in these cultures. The data also indicated that a transcarboxylation reaction between succinate and propionyl coenzyme A occurred, yielding propionate and methylmalonyl coenzyme A.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boone D. R., Bryant M. P. Propionate-Degrading Bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from Methanogenic Ecosystems. Appl Environ Microbiol. 1980 Sep;40(3):626–632. doi: 10.1128/aem.40.3.626-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boone D. R. Mixed-culture fermentor for simulating methanogenic digestors. Appl Environ Microbiol. 1984 Jul;48(1):122–126. doi: 10.1128/aem.48.1.122-126.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boone D. R. Propionate exchange reactions in methanogenic ecosystems. Appl Environ Microbiol. 1984 Oct;48(4):863–864. doi: 10.1128/aem.48.4.863-864.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boone D. R. Terminal reactions in the anaerobic digestion of animal waste. Appl Environ Microbiol. 1982 Jan;43(1):57–64. doi: 10.1128/aem.43.1.57-64.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KAZIRO Y., OCHOA S. THE METABOLISM OF PROPIONIC ACID. Adv Enzymol Relat Areas Mol Biol. 1964;26:283–378. doi: 10.1002/9780470122716.ch7. [DOI] [PubMed] [Google Scholar]
  6. Kaspar H. F., Wuhrmann K. Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge. Appl Environ Microbiol. 1978 Jul;36(1):1–7. doi: 10.1128/aem.36.1.1-7.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koch M., Dolfing J., Wuhrmann K., Zehnder A. J. Pathways of propionate degradation by enriched methanogenic cultures. Appl Environ Microbiol. 1983 Apr;45(4):1411–1414. doi: 10.1128/aem.45.4.1411-1414.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mackie R. I., Bryant M. P. Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO(2) to Methanogenesis in Cattle Waste at 40 and 60 degrees C. Appl Environ Microbiol. 1981 Jun;41(6):1363–1373. doi: 10.1128/aem.41.6.1363-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robbins J. E., Armold M. T., Weiel J. E., Runquist E. A. Stabilizing effect of acetate salts and HCO-3 in digestors receiving high levels of glucose. Can J Microbiol. 1983 Oct;29(10):1405–1411. doi: 10.1139/m83-216. [DOI] [PubMed] [Google Scholar]

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