Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Sep;53(9):2262–2264. doi: 10.1128/aem.53.9.2262-2264.1987

New selective and differential medium for Vibrio cholerae and Vibrio vulnificus.

G Massad 1, J D Oliver 1
PMCID: PMC204093  PMID: 3674873

Abstract

Thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for Vibrio cholerae and Vibrio vulnificus is inadequate. Therefore, a new plating medium, cellobiose-polymyxin B-colistin agar, was developed for the isolation of these two species. Cellobiose-polymyxin B-colistin agar demonstrated a significant advantage over other media designed for the isolation or differentiation of vibrios: of both the 136 strains representing 19 Vibrio species and the marine isolates of the genera Pseudomonas, Flavobacterium, and Photobacterium, only V. vulnificus and V. cholerae were able to grow. Furthermore, the fermentation of cellobiose by V. vulnificus allowed for the easy differentiation of these two species. This medium offers significant potential as a selective and differential medium for these two pathogenic vibrios.

Full text

PDF
2262

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  2. Brayton P. R., West P. A., Russek E., Colwell R. R. New selective plating medium for isolation of Vibrio vulnificus biogroup 1. J Clin Microbiol. 1983 Jun;17(6):1039–1044. doi: 10.1128/jcm.17.6.1039-1044.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colwell R. R., Seidler R. J., Kaper J., Joseph S. W., Garges S., Lockman H., Maneval D., Bradford H., Roberts N., Remmers E. Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries. Appl Environ Microbiol. 1981 Feb;41(2):555–558. doi: 10.1128/aem.41.2.555-558.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kaper J., Lockman H., Colwell R. R., Joseph S. W. Ecology, serology, and enterotoxin production of Vibrio cholerae in Chesapeake Bay. Appl Environ Microbiol. 1979 Jan;37(1):91–103. doi: 10.1128/aem.37.1.91-103.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Karunasagar I., Venugopal M. N., Karunasagar I., Segar K. Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood. Appl Environ Microbiol. 1986 Sep;52(3):583–585. doi: 10.1128/aem.52.3.583-585.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly M. T. Effect of temperature and salinity on Vibrio (Beneckea) vulnificus occurrence in a Gulf Coast environment. Appl Environ Microbiol. 1982 Oct;44(4):820–824. doi: 10.1128/aem.44.4.820-824.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morris G. K., Merson M. H., Huq I., Kibrya A. K., Black R. Comparison of four plating media for isolating Vibrio cholerae. J Clin Microbiol. 1979 Jan;9(1):79–83. doi: 10.1128/jcm.9.1.79-83.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oliver J. D., Warner R. A., Cleland D. R. Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment. Appl Environ Microbiol. 1983 Mar;45(3):985–998. doi: 10.1128/aem.45.3.985-998.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rennels M. B., Levine M. M., Daya V., Angle P., Young C. Selective vs. nonselective media and direct plating vs. enrichment technique in isolation of Vibrio cholerae: recommendations for clinical laboratories. J Infect Dis. 1980 Sep;142(3):328–331. doi: 10.1093/infdis/142.3.328. [DOI] [PubMed] [Google Scholar]
  10. Tacket C. O., Brenner F., Blake P. A. Clinical features and an epidemiological study of Vibrio vulnificus infections. J Infect Dis. 1984 Apr;149(4):558–561. doi: 10.1093/infdis/149.4.558. [DOI] [PubMed] [Google Scholar]
  11. Tilton R. C., Ryan R. W. Clinical and ecological characteristics of Vibrio vulnificus in the northeastern United States. Diagn Microbiol Infect Dis. 1987 Feb;6(2):109–117. doi: 10.1016/0732-8893(87)90094-0. [DOI] [PubMed] [Google Scholar]
  12. Twedt R. M., Madden J. M., Hunt J. M., Francis D. W., Peeler J. T., Duran A. P., Hebert W. O., McCay S. G., Roderick C. N., Spite G. T. Characterization of Vibrio cholerae isolated from oysters. Appl Environ Microbiol. 1981 Jun;41(6):1475–1478. doi: 10.1128/aem.41.6.1475-1478.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. West P. A., Russek E., Brayton P. R., Colwell R. R. Statistical evaluation of a quality control method for isolation of pathogenic Vibrio species on selected thiosulfate-citrate-bile salts-sucrose agars. J Clin Microbiol. 1982 Dec;16(6):1110–1116. doi: 10.1128/jcm.16.6.1110-1116.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES