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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1984 Apr;65(2):201–214.

A model of persistent antigen-induced chronic inflammation in the rat air pouch.

S Yoshino, P A Bacon, D R Blake, D L Scott, A C Wainwright, K W Walton
PMCID: PMC2040961  PMID: 6370290

Abstract

Continuing antigen-induced inflammation was established in a subcutaneous air pouch in rats by recurrent local challenge. The animals were sensitized using bovine serum albumin in Freund's complete adjuvant and were challenged 14 days later by injection of the antigen in a solution containing sodium carboxymethylcellulose into the air pouch to produce allergic inflammation. A single antigenic challenge induced acute inflammation with a predominantly polymorph infiltration in the first 48 h. Later samples showed a low-grade mononuclear response which persisted for 4-5 days. Repeated challenge produced chronic inflammation with an accentuated mononuclear response. Connective tissue activation involving fibronectin and collagen was seen as the inflammation progressed, and this was associated with production of ferritin by mononuclear cells. Discontinuation of challenge injections resulted in resolution of the granuloma. We suggest this model can be used to investigate the mechanisms involved in chronic inflammatory diseases with an immunological component and to evaluate the effects of therapeutic intervention upon chronic allergic inflammation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Edwards J. C., Sedgwick A. D., Willoughby D. A. The formation of a structure with the features of synovial lining by subcutaneous injection of air: an in vivo tissue culture system. J Pathol. 1981 Jun;134(2):147–156. doi: 10.1002/path.1711340205. [DOI] [PubMed] [Google Scholar]
  2. Glynn L. E. The chronicity of inflammation and its significance in rheumatoid arthritis. Ann Rheum Dis. 1968 Mar;27(2):105–121. doi: 10.1136/ard.27.2.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gordon H., Sweets H. H. A Simple Method for the Silver Impregnation of Reticulum. Am J Pathol. 1936 Jul;12(4):545–552.1. [PMC free article] [PubMed] [Google Scholar]
  4. Kurkinen M., Vaheri A., Roberts P. J., Stenman S. Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest. 1980 Jul;43(1):47–51. [PubMed] [Google Scholar]
  5. LENDRUM A. C., FRASER D. S., SLIDDERS W., HENDERSON R. Studies on the character and staining of fibrin. J Clin Pathol. 1962 Sep;15:401–413. doi: 10.1136/jcp.15.5.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Muirden K. D. Ferritin in synovial cells in patients with rheumatoid arthritis. Ann Rheum Dis. 1966 Sep;25(5):387–401. doi: 10.1136/ard.25.5.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ohuchi K., Yoshino S., Kanaoka K., Tsurufuji S., Levine L. A possible role of arachidonate metabolism in allergic air pouch inflammation in rats. Anti-inflammatory effect of indomethacin and dexamethasone and the level of prostaglandin E2 in the exudate. Int Arch Allergy Appl Immunol. 1982;68(4):326–331. doi: 10.1159/000233121. [DOI] [PubMed] [Google Scholar]
  8. Ohuchi K., Yoshino S., Kurihara A., Yoshimura H., Ishiguro M., Kiso S., Tsurufuji S. Delayed-type hypersensitivity as revealed on the footpads of mice to azobenzenearsonate-acetyl bovine serum albumin. Int Arch Allergy Appl Immunol. 1981;66(4):391–403. doi: 10.1159/000232848. [DOI] [PubMed] [Google Scholar]
  9. PEARSON C. M. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc Soc Exp Biol Med. 1956 Jan;91(1):95–101. doi: 10.3181/00379727-91-22179. [DOI] [PubMed] [Google Scholar]
  10. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SELYE H. On the mechanism through which hydrocortisone affects the resistance of tissues to injury; an experimental study with the granuloma pouch technique. J Am Med Assoc. 1953 Jul 25;152(13):1207–1213. doi: 10.1001/jama.1953.63690130001006. [DOI] [PubMed] [Google Scholar]
  12. Sato H., Hashimoto M., Sugio K., Ohuchi K., Tsurufuji S. Comparative study between steroidal and nonsteroidal anti-inflammatory drugs on the mode of their actions on vascular permeability in rat carrageenin-air-pouch inflammation. J Pharmacobiodyn. 1980 Jul;3(7):345–352. doi: 10.1248/bpb1978.3.345. [DOI] [PubMed] [Google Scholar]
  13. Schofield B. H., Williams B. R., Doty S. B. Alcian Blue staining of cartilage for electron microscopy. Application of the critical electrolyte concentation principle. Histochem J. 1975 Mar;7(2):139–149. doi: 10.1007/BF01004558. [DOI] [PubMed] [Google Scholar]
  14. Scott D. L., Bedford P. A., Walton K. W. The preparation of plasma fibronectin antigen and antiserum. J Immunol Methods. 1981;43(1):29–33. doi: 10.1016/0022-1759(81)90033-8. [DOI] [PubMed] [Google Scholar]
  15. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  16. Trentham D. E., Townes A. S., Kang A. H. Autoimmunity to type II collagen an experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857–868. doi: 10.1084/jem.146.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Urbaniak S. J., Greiss M. A., Crawford R. J., Fergusson M. C. Prediction of the severity of rhesus haemolytic disease of the newborn by an ADCC assay. Lancet. 1981 Jul 18;2(8238):142–143. doi: 10.1016/s0140-6736(81)90316-0. [DOI] [PubMed] [Google Scholar]

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