Abstract
The role of granulocytes in the induction of endocarditis with a dextran-producing Streptococcus sanguis and a dextran-negative mutant of this strain was studied. The number of colony-forming units of Streptococcus sanguis needed to colonize the vegetations in 50% of the rabbits (ID50) was significantly lower for the parent strain than for the dextran-negative mutant. However, in granulocytopenic rabbits the ID50s of both strains did not differ measurably. Dextran-negative streptococci were more readily cleared from the circulation than dextran-positive, but in this respect no difference was found between control and granulocytopenic rabbits, which indicates that clearance cannot account for the difference in ID50 between the two strains in the control group. At serum concentrations of 5% and lower, in-vitro granulocytes phagocytosed the dextran-negative streptococci more rapidly than the dextran-positive. The intracellular killing of the streptococci was no influenced by dextran production. This study suggests that an impaired phagocytic removal of attached bacteria from the vegetational surface can be a factor promoting the induction of endocarditis by dextran-producing streptococci.
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Selected References
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- ANGRIST A. A., OKA M. Pathogenesis of bacterial endocarditis. JAMA. 1963 Jan 26;183:249–252. doi: 10.1001/jama.1963.63700040009010b. [DOI] [PubMed] [Google Scholar]
- Durack D. T., Beeson P. B. Experimental bacterial endocarditis. I. Colonization of a sterile vegetation. Br J Exp Pathol. 1972 Feb;53(1):44–49. [PMC free article] [PubMed] [Google Scholar]
- Gould K., Ramirez-Ronda C. H., Holmes R. K., Sanford J. P. Adherence of bacteria to heart valves in vitro. J Clin Invest. 1975 Dec;56(6):1364–1370. doi: 10.1172/JCI108216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leijh P. C., van den Barselaar M. T., Dubbeldeman-Rempt I., van Furth R. Kinetics of intracellular killing of Staphylococcus aureus and Escherichia coli by human granulocytes. Eur J Immunol. 1980 Oct;10(10):750–757. doi: 10.1002/eji.1830101005. [DOI] [PubMed] [Google Scholar]
- Meddens M. J., Thompson J., Eulderink F., Bauer W. C., Mattie H., van Furth R. Role of granulocytes in experimental Streptococcus sanguis endocarditis. Infect Immun. 1982 Apr;36(1):325–332. doi: 10.1128/iai.36.1.325-332.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pelletier L. L., Jr, Coyle M., Petersdorf R. Dextran production as a possible virulence factor in streptococcal endocarditis. Proc Soc Exp Biol Med. 1978 Jul;158(3):415–420. doi: 10.3181/00379727-158-40216. [DOI] [PubMed] [Google Scholar]
- Ramirez-Ronda C. H. Adherence of glucan-positive and glucan-negative streptococcal strains to normal and damaged heart valves. J Clin Invest. 1978 Oct;62(4):805–814. doi: 10.1172/JCI109192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheld W. M., Valone J. A., Sande M. A. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. doi: 10.1172/JCI109057. [DOI] [PMC free article] [PubMed] [Google Scholar]