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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1985 Feb;66(1):79–87.

Varying expression of major histocompatibility complex antigens on human renal endothelium and epithelium.

P R Evans, L P Trickett, J L Smith, A G MacIver, D Tate, M Slapak
PMCID: PMC2041029  PMID: 3855644

Abstract

Pre-anastomosis wedge biopsies from 14 cadaveric donor kidneys were examined for the expression of class I (HLA-ABC) and class II (HLA-DR) antigens in renal tissue. Two monoclonal antibodies to class I antigens and four to class II antigens were used in an indirect immunoperoxidase technique. Consistent expression of both antigens was demonstrated on the surface of glomerular, peritubular capillary and venous endothelial cells. Renal arteries contained only class I antigens. Proximal tubules contained varying amounts of each antigen in their cytoplasm. Sixteen human lymphocytotoxic allo-antisera showed marked variation in their ability to detect HLA antigens on the kidney. The selection of donors for recipients of renal allografts involves the complement-dependent cytotoxicity test and the failure of some lymphocytotoxic antisera to bind to the kidney indicates that some suitable patients may be incorrectly excluded. The use of a binding assay using an immunoperoxidase technique should be included in cross-match techniques particularly for patients who have high levels of circulating cytotoxic antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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