Abstract
Several chimpanzees found to be seronegative for hepatitis A by immune electron microscopy and by radioimmunoassay were inoculated with known infective faecal extracts from several sources, including the MS-1 strain of hepatitis A virus, first passage material from chimpanzee George and a strain of hepatitis A virus obtained during a naturally occurring outbreak of infection in Germany. Elevated serum transaminase levels were found in the chimpanzees about 19 days after inoculation. Virus particles were found in faeces as early as 9 days after infection in one chimpanzee and by days 12 and 14 in the others. Excretion of virus continued for 9 to 19 days, and the maximum numbers of virus particles were found on the 17th to the 19th day after inoculation. The buoyant density in caesium chloride of virus particles separated from faeces was 1.31 to 1.43 g/ml. Most of the particles were found in the fraction with a density of 1.34 g/ml.
Hepatitis A antibody was detected in the serum by immune electron microscopy and by radioimmunoassay during the period of incubation while virus particles were still being excreted in the faeces and coinciding approximately with the onset of biochemical evidence of liver damage.
A very close or identical morphological and serological relationship was demonstrated between hepatitis A virus isolated from man and from the experimentally infected chimpanzees.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boggs J. D., Melnick J. L., Conrad M. E., Felsher B. F. Viral hepatitis. Clinical and tissue culture studies. JAMA. 1970 Nov 9;214(6):1041–1046. doi: 10.1001/jama.214.6.1041. [DOI] [PubMed] [Google Scholar]
- Bradley D. W., Hornbeck C. L., Cood E. H., Maynard J. E., Gravelle C. R. CsCl banding of hepatitis A-associated virus-like particles. J Infect Dis. 1975 Mar;131(3):304–306. doi: 10.1093/infdis/131.3.304. [DOI] [PubMed] [Google Scholar]
- Dienstag J. L., Alling D. W., Purcell R. H. Quantitation of antibody to hepatitis A antigen by immune electron microscopy. Infect Immun. 1976 Apr;13(4):1209–1213. doi: 10.1128/iai.13.4.1209-1213.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dienstag J. L., Feinstone S. M., Purcell R. H., Hoofnagle J. H., Barker L. F., London W. T., Popper H., Peterson J. M., Kapikian A. Z. Experimental infection of chimpanzees with hepatitis A virus. J Infect Dis. 1975 Nov;132(5):532–545. doi: 10.1093/infdis/132.5.532. [DOI] [PubMed] [Google Scholar]
- Feinstone S. M., Kapikian A. Z., Gerin J. L., Purcell R. H. Buoyant density of the hepatitis A virus-like particle in cesium chloride. J Virol. 1974 Jun;13(6):1412–1414. doi: 10.1128/jvi.13.6.1412-1414.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
- Gravelle C. R., Hornbeck C. L., Maynard J. E., Schable C. A., Cook E. H., Bradley D. W. Hepatitis A: report of a common-source outbreak with recovery of a possible etiologic agent. II. Laboratory studies. J Infect Dis. 1975 Feb;131(2):167–171. doi: 10.1093/infdis/131.2.167. [DOI] [PubMed] [Google Scholar]
- Maynard J. E., Bradley D. W., Gravelle C. R., Ebert J. W., Krushak D. H. Preliminary studies of hepatitis A in chimpanzees. J Infect Dis. 1975 Feb;131(2):194–197. doi: 10.1093/infdis/131.2.194. [DOI] [PubMed] [Google Scholar]
- Provost P. J., Wolanski B. S., Miller W. J., Ittensohn O. L., McAleer W. J., Hilleman M. R. Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578). Proc Soc Exp Biol Med. 1975 Feb;148(2):532–539. doi: 10.3181/00379727-148-38578. [DOI] [PubMed] [Google Scholar]
- Thornton A., Zuckerman A. J., Almeida J. D. Letter: Chimpanzee model for hepatitis-A virus. Lancet. 1975 Aug 2;2(7927):226–226. doi: 10.1016/s0140-6736(75)90692-3. [DOI] [PubMed] [Google Scholar]