Skip to main content
NCBI home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1976 Dec;57(6):733–741.

Inhibition of the liver and plasma protein acute-phase response in mice by D-galactosamine.

A Koj, A Dubin
PMCID: PMC2041238  PMID: 12781

Abstract

Local inflammation evoked in Swiss albino mice by subcutaneous injection of Celite resulted in a rise of liver tyrosine aminotransferase activity and plasma level of fibrinogen and seromucoid, while liver alanine aminotransferase activity and plasma level of fibrinogen and seromucoid, while liver alanine aminotransferase activity and the plasma level of albumin and total protein remained unaltered. By measuring the incorporation of [14C] leucine, stimulation of liver and plasms protein synthesis by Celite injection was demonstrated. Administration of D-galactosamine (2-5 mg/10 g body weight) inhibited the enhanced synthesis of liver proteins, and especially of trauma-induced synthesis of plasma fibrinogen and seromucoid. The inhibitory effect of galactosamine was most pronounced when the amino sugar was injected simultaneously with Celite and then protein synthesis was measured 6 h later. The results obtained support the idea that high doses of galactosamine inhibit transcription of trauma-inducible mRNA in the liver and thus block the acute-phase response.

Full text

PDF
733

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anukarahanonta T., Shinozuka H., Farber E. Inhibition of protein synthesis in rat liver by D-galactosamine. Res Commun Chem Pathol Pharmacol. 1973 Mar;5(2):481–491. [PubMed] [Google Scholar]
  2. Balegno H. F., Neuhaus O. W. Effect of insulin on the injury-stimulated synthesis of serum albumin in the rat. Life Sci II. 1970 Sep 22;9(18):1039–1044. doi: 10.1016/0024-3205(70)90012-3. [DOI] [PubMed] [Google Scholar]
  3. Bauer C. H., Lukaschek R., Reutter W. G. Studies on the golgi apparatus. Cumulative inhibition of protein and glycoprotein secretion by D-galactosamine. Biochem J. 1974 Aug;142(2):221–230. doi: 10.1042/bj1420221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geller E., Yuwiler A., Schapiro S. Tyrosine aminotransferase: activation or repression by a stress. Proc Soc Exp Biol Med. 1969 Feb;130(2):458–461. doi: 10.3181/00379727-130-33579. [DOI] [PubMed] [Google Scholar]
  5. Keppler D. O., Pausch J., Decker K. Selective uridine triphosphate deficiency induced by D-galactosamine in liver and reversed by pyrimidine nucleotide precursors. Effect on ribonucleic acid synthesis. J Biol Chem. 1974 Jan 10;249(1):211–216. [PubMed] [Google Scholar]
  6. Keppler D. O., Rudigier J. F., Bischoff E., Decker K. F. The trapping of uridine phosphates by D-galactosamine. D-glucosamine, and 2-deoxy-D-galactose. A study on the mechanism of galactosamine hepatitis. Eur J Biochem. 1970 Dec;17(2):246–253. doi: 10.1111/j.1432-1033.1970.tb01160.x. [DOI] [PubMed] [Google Scholar]
  7. Keppler D., Decker K. Studies on the mechanism of galactosamine-1-phosphate and its inhibition of UDP-glucose pyrophosphorylase. Eur J Biochem. 1969 Sep;10(2):219–225. doi: 10.1111/j.1432-1033.1969.tb00677.x. [DOI] [PubMed] [Google Scholar]
  8. Kinjitsukada, Oura H., Nakashima S., Hayasaki N. Adaptive response of tryptophan pyrrolase and tyrosine transaminase in rat liver after partial hepatectomy and laparotomy. Biochim Biophys Acta. 1968 Sep 3;165(2):218–224. doi: 10.1016/0304-4165(68)90049-4. [DOI] [PubMed] [Google Scholar]
  9. Koj A., Dubin A. On the hormonal modulation of acute-phase plasma protein synthesis in perfused rat liver. Acta Biochim Pol. 1974;21(2):159–167. [PubMed] [Google Scholar]
  10. Koj A., McFarlane A. S. Effect of endotoxin on plasma albumin and fibrinogen synthesis rates in rabbits as measured by the [14C] carbonate method. Biochem J. 1968 Jun;108(1):137–146. doi: 10.1042/bj1080137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koj A. The measurement of absolute and relative synthesis rates of fibrinogen in vivo. Biochim Biophys Acta. 1968 Aug 6;165(1):97–107. doi: 10.1016/0304-4165(68)90193-1. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Neuhaus O. W., Balegno H. F., Chandler A. M. Induction of plasma protein synthesis in response to trauma. Am J Physiol. 1966 Jul;211(1):151–156. doi: 10.1152/ajplegacy.1966.211.1.151. [DOI] [PubMed] [Google Scholar]
  14. Reynolds R. D., Reutter W. Inhibition of induction of rat liver tyrosine aminotransferase by D-galactosamine. J Biol Chem. 1973 Mar 10;248(5):1562–1567. [PubMed] [Google Scholar]
  15. WINZLER R. J. Determination of serum glycoproteins. Methods Biochem Anal. 1955;2:279–311. doi: 10.1002/9780470110188.ch10. [DOI] [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES