Skip to main content
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1977 Apr;58(2):160–167.

The effects of long-term exposure of lung fibroblast strains to chrysotile asbestos.

P M Hext, J Hunt, K S Dodgson, R J Richards
PMCID: PMC2041284  PMID: 861166

Abstract

Evidence is presented that persistent long-term exposure of a strain of lung fibroblast-like cells to chrysotile asbestos over 37 passages in vitro leads to enhancement in cell mat collagen deposition and is accompanied by other metabolic alterations in the cultures.

Full text

PDF
160

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CERIOTTI G. Determination of nucleic acids in animal tissues. J Biol Chem. 1955 May;214(1):59–70. [PubMed] [Google Scholar]
  3. Hext P. M., Richards R. J. Biochemical effects of asbestiform minerals on lung fibroblast cultures. Br J Exp Pathol. 1976 Jun;57(3):281–285. [PMC free article] [PubMed] [Google Scholar]
  4. Kurtz M. J., Stidworthy G. H. Control over acid mucopolysaccharide production of cells in vitro. Biochim Biophys Acta. 1975 Jul 14;399(1):90–100. doi: 10.1016/0304-4165(75)90215-9. [DOI] [PubMed] [Google Scholar]
  5. Motomiya M., Arai H., Sato H., Yokosawa A., Nagai H. Increase of dermatan sulfate in a case of pulmonary fibrosis. Tohoku J Exp Med. 1975 Apr;115(4):361–365. doi: 10.1620/tjem.115.361. [DOI] [PubMed] [Google Scholar]
  6. OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
  7. Obrink B. The influence of glycosaminoglycans on the formation of fibers from monomeric tropocollagen in vitro. Eur J Biochem. 1973 Apr 2;34(1):129–137. doi: 10.1111/j.1432-1033.1973.tb02739.x. [DOI] [PubMed] [Google Scholar]
  8. Oegema T. R., Jr, Laidlaw J., Hascall V. C., Dziewiatkowski D. D. The effect of proteoglycans on the formation of fibrils from collagen solutions. Arch Biochem Biophys. 1975 Oct;170(2):698–709. doi: 10.1016/0003-9861(75)90167-8. [DOI] [PubMed] [Google Scholar]
  9. Richards G. M. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem. 1974 Feb;57(2):369–376. doi: 10.1016/0003-2697(74)90091-8. [DOI] [PubMed] [Google Scholar]
  10. Richards R. J., Hext P. M., Blundell G., Henderson W. J., Volcani B. E. Ultrastructural changes in lung fibroblast cultures exposed to chrysotile asbestos. Br J Exp Pathol. 1974 Jun;55(3):275–281. [PMC free article] [PubMed] [Google Scholar]
  11. Richards R. J., Jacoby F. Light microscope studies on the effects of chrysotile asbestos and fiber glass on the morphology and reticulin formation of cultured lung fibroblasts. Environ Res. 1976 Feb;11(1):112–121. doi: 10.1016/0013-9351(76)90114-6. [DOI] [PubMed] [Google Scholar]
  12. Richards R. J., Wusteman F. S., Dodgson K. S. The direct effects of dusts on lung fibroblasts grown in vitro. Life Sci I. 1971 Oct 15;10(20):1149–1159. doi: 10.1016/0024-3205(71)90275-x. [DOI] [PubMed] [Google Scholar]

Articles from British journal of experimental pathology are provided here courtesy of Wiley

RESOURCES