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Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Oct;53(10):2583–2587. doi: 10.1128/aem.53.10.2583-2587.1987

Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts

Steven A Woskow 1,, Jeffery K Kondo 1,*
PMCID: PMC204149  PMID: 16347474

Abstract

With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 104 to 105 transformants and transfectants per μg of DNA were achieved. The procedure was used to transform protoplasts of Streptococcus cremoris CS224 at low frequency (5 transformants per μg of DNA).

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Selected References

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