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. 1987 Oct;53(10):2588–2589. doi: 10.1128/aem.53.10.2588-2589.1987

Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

J A Lippke 1, M N Strzempko 1, F F Raia 1, S L Simon 1, C K French 1
PMCID: PMC204150  PMID: 3426222

Abstract

A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.

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Selected References

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  1. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]

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