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. 1987 Oct;53(10):2590–2592. doi: 10.1128/aem.53.10.2590-2592.1987

Freeze-Thawing of Aquaspirillum magnetotacticum Cells Selectively Releases Periplasmic Proteins

Lawrence C Paoletti 1, Kevin A Short 1, Nancy Blakemore 1, Richard P Blakemore 1,*
PMCID: PMC204151  PMID: 16347475

Abstract

Cells of the gram-negative bacterium Aquaspirillum magnetotacticum, when suspended in buffer and freeze-thawed, produced pinkish orange supernatant fluid. The fluid contained ≤2.0% of total extractable outer membrane component 2-keto-3-deoxyoctonate or of the cytoplasmic membrane marker succinic dehydrogenase. Electrophoretic banding patterns and difference spectra of proteins and hemoproteins released by freeze-thawing cells were distinct from those of membrane-associated substances and similar to those of periplasmic substances obtained by applying conventional fractionation methods to this organism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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