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. 1987 Oct;53(10):2593–2596. doi: 10.1128/aem.53.10.2593-2596.1987

Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.

P K Jepsen 1, E Riise 1, K Biedermann 1, P C Kristensen 1, C Emborg 1
PMCID: PMC204152  PMID: 3322193

Abstract

Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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