Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Oct;53(10):2600–2602. doi: 10.1128/aem.53.10.2600-2602.1987

Citrate Cycle Intermediates in the Metabolism of Aspartate and Lactate by Propionibacterium freudenreichii subsp. shermanii

Vaughan L Crow 1
PMCID: PMC204154  PMID: 16347477

Abstract

Propionibacterium freudenreichii subsp. shermanii metabolized 7 mol of aspartate to 6 mol of succinate, 4 mol of CO2, and 7 mol of ammonia. When lactate, sparged with 100% CO2, was fermented at pH 5.5, unexpectedly high ratios of propionate to acetate were obtained (i.e., 3.2 to 3.8:1). Citrate cycle intermediates may be involved in these fermentations.

Full text

PDF
2600

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Crow V. L. Metabolism of Aspartate by Propionibacterium freudenreichii subsp. shermanii: Effect on Lactate Fermentation. Appl Environ Microbiol. 1986 Aug;52(2):359–365. doi: 10.1128/aem.52.2.359-365.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crow V. L., Thomas T. D. Arginine metabolism in lactic streptococci. J Bacteriol. 1982 Jun;150(3):1024–1032. doi: 10.1128/jb.150.3.1024-1032.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crow V. L. Utilization of Lactate Isomers by Propionibacterium freudenreichii subsp. shermanii: Regulatory Role for Intracellular Pyruvate. Appl Environ Microbiol. 1986 Aug;52(2):352–358. doi: 10.1128/aem.52.2.352-358.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fordyce A. M., Crow V. L., Thomas T. D. Regulation of product formation during glucose or lactose limitation in nongrowing cells of Streptococcus lactis. Appl Environ Microbiol. 1984 Aug;48(2):332–337. doi: 10.1128/aem.48.2.332-337.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SIU P. M., WOOD H. G. Phosphoenolpyruvic carboxytransphosphorylase, a CO2 fixation enzyme from propionic acid bacteria. J Biol Chem. 1962 Oct;237:3044–3051. [PubMed] [Google Scholar]
  7. Thomas T. D., Ellwood D. C., Longyear V. M. Change from homo- to heterolactic fermentation by Streptococcus lactis resulting from glucose limitation in anaerobic chemostat cultures. J Bacteriol. 1979 Apr;138(1):109–117. doi: 10.1128/jb.138.1.109-117.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. WOOD H. G., STJERNHOLM R., LEAVER F. W. The metabolism of labeled glucose by the propionic acid bacteria. J Bacteriol. 1955 Nov;70(5):510–520. doi: 10.1128/jb.70.5.510-520.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wood H. G. Metabolic cycles in the fermentation by propionic acid bacteria. Curr Top Cell Regul. 1981;18:255–287. doi: 10.1016/b978-0-12-152818-8.50021-9. [DOI] [PubMed] [Google Scholar]
  10. Wood H. G., Werkman C. H. Mechanism of glucose dissimilation by the propionic acid bacteria. Biochem J. 1936 Apr;30(4):618–623. doi: 10.1042/bj0300618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wood H. G., Werkman C. H. The relationship of bacterial utilization of CO(2) to succinic acid formation. Biochem J. 1940 Feb;34(2):129–138. doi: 10.1042/bj0340129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wood H. G., Werkman C. H. The utilization of CO(2) by the propionic acid bacteria. Biochem J. 1938 Jul;32(7):1262–1271. doi: 10.1042/bj0321262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Vries W., van Wyck-Kapteyn W. M., Stouthamer A. H. Generation of ATP during cytochrome-linked anaerobic electron transport in propionic acid bacteria. J Gen Microbiol. 1973 May;76(1):31–41. doi: 10.1099/00221287-76-1-31. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES