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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1980 Feb;61(1):108–116.

Effects of monocytopenia and anticoagulation in experimental Streptococcus sanguis endocarditis.

L Thörig, J Thompson, F Eulderink, J J Emeis, R Van Furth
PMCID: PMC2041555  PMID: 7378272

Abstract

The role of blood monocytes in the attachment of streptococci to endocardial vegetations was investigated in an experimental Streptococcus sanguis endocarditis by depletion of blood monocytes with the cytostatic drug VP 16-213 alone and combined with anticoagulant treatment with warfarin sodium. The numbers of streptococci in the vegetations of control, monocytopenic, and monocytopenic/anticoagulated rabbits were comparable. In the vegetations streptococci were found mainly in areas free of phagocytic cells. It is concluded that streptococci do not have to be phagocytosed by monocytes in the circulation before being deposited on the surface of endocardial vegetations. Even the vegetations of intensively anticoagulated/monocytopenic rabbits showed colonies of streptococci embedded in polymerized fibrin and cellular material, this matrix possibly being held together by streptococcal dextran.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bleyl U., Sebening H., Kuhn W. Morphologischer Nachweis von Fibrinmonomeren im histologischen Schnitt. Thromb Diath Haemorrh. 1969 Aug 31;22(1):68–86. [PubMed] [Google Scholar]
  2. Dombernowsky P., Nissen N. I., Larsen V. Clinical investigation of a new podophyllum derivative, epipodophyllotoxin, 4'-demethyl-9-(4,6-O-2-thenylidene- -D-glucopyranoside) (NSC-122819), in patients with malignant lymphomas and solid tumors. Cancer Chemother Rep. 1972 Feb;56(1):71–82. [PubMed] [Google Scholar]
  3. Durack D. T., Beeson P. B. Experimental bacterial endocarditis. I. Colonization of a sterile vegetation. Br J Exp Pathol. 1972 Feb;53(1):44–49. [PMC free article] [PubMed] [Google Scholar]
  4. Durack D. T. Experimental bacterial endocarditis. IV. Structure and evolution of very early lesions. J Pathol. 1975 Feb;115(2):81–89. doi: 10.1002/path.1711150204. [DOI] [PubMed] [Google Scholar]
  5. Hoak J. C., Connor W. E., Warner E. D. The antithrombotic effects of sodium heparin and sodium warfarin. Arch Intern Med. 1966 Jan;117(1):25–31. [PubMed] [Google Scholar]
  6. Morris G. K., Mitchell J. R. Warfarin sodium in prevention of deep venous thrombosis and pulmonary embolism in patients with fractured neck of femur. Lancet. 1976 Oct 23;2(7991):869–872. doi: 10.1016/s0140-6736(76)90536-5. [DOI] [PubMed] [Google Scholar]
  7. Pelletier L. L., Jr, Coyle M., Petersdorf R. Dextran production as a possible virulence factor in streptococcal endocarditis. Proc Soc Exp Biol Med. 1978 Jul;158(3):415–420. doi: 10.3181/00379727-158-40216. [DOI] [PubMed] [Google Scholar]
  8. Ramirez-Ronda C. H. Adherence of glucan-positive and glucan-negative streptococcal strains to normal and damaged heart valves. J Clin Invest. 1978 Oct;62(4):805–814. doi: 10.1172/JCI109192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rozencweig M., Von Hoff D. D., Henney J. E., Muggia F. M. VM 26 and VP 16-213: a comparative analysis. Cancer. 1977 Jul;40(1):334–342. doi: 10.1002/1097-0142(197707)40:1<334::aid-cncr2820400147>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  10. Scheld W. M., Valone J. A., Sande M. A. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. doi: 10.1172/JCI109057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thompson J., Eulderink F., Lemkes H., van Furth R. Effect of warfarin on the induction and course of experimental endocarditis. Infect Immun. 1976 Dec;14(6):1284–1289. doi: 10.1128/iai.14.6.1284-1289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weinstein L., Rubin R. H. Infective endocarditis--1973. Prog Cardiovasc Dis. 1973 Nov-Dec;16(3):239–274. doi: 10.1016/s0033-0620(73)80001-5. [DOI] [PubMed] [Google Scholar]
  13. Weinstein L., Schlesinger J. J. Pathoanatomic, pathophysiologic and clinical correlations in endocarditis (first of two parts). N Engl J Med. 1974 Oct 17;291(16):832–837. doi: 10.1056/NEJM197410172911609. [DOI] [PubMed] [Google Scholar]
  14. van Ginkel C. J., Thörig L., Thompson J., Oh J. I., van Aken W. G. Enhancement of generation of monocyte tissue thromboplastin by bacterial phagocytosis: possible pathway for fibrin formation on infected vegetations in bacterial endocarditis. Infect Immun. 1979 Jul;25(1):388–395. doi: 10.1128/iai.25.1.388-395.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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