Abstract
Comparison of cotton wool and sponge-induced granulomas in the rat showed that collagen contents were very similar despite the different pattern of granuloma formation. There was significantly less variation of individual results in the sponge. Although collagen accounted for less than 10% of the dry weight, there was a positive correlation between the 2 parameters in both models of 9 days but only in the pellet at 15 days. Hydrocortisone treatment significantly decreased dry weight and collagen content. There was rapid cell proliferation and collagen synthesis in the sponge granuloma from Day 5, reaching a peak between the ninth and twelfth day, when DNA levels dropped and collagen increased more slowly to plateau by Day 15. Day 9 was chosen as the most suitable stage to study drug effects. Dexamethasone significantly decreased collagen synthesis whereas indomethacin and phenylbutazone did not. Soluble collagen levels were increased with beta-amino-propionitrile and d-penicillamine; a dose-related effect was obtained with d-penicillamine.
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bailey A. J., Bazin S., Delaunay A. Changes in the nature of the collagen during development and resorption of granulatin tissue. Biochim Biophys Acta. 1973 Dec 6;328(2):383–390. doi: 10.1016/0005-2795(73)90272-9. [DOI] [PubMed] [Google Scholar]
- Chvapil M. Pharmacology of fibrosis: definitions, limits and perspectives. Life Sci. 1975 May 1;16(9):1345–1361. doi: 10.1016/0024-3205(75)90031-4. [DOI] [PubMed] [Google Scholar]
- Cutroneo K. R., Costello D., Fuller G. C. Alteration of proline hydroxylase activity by glucocorticoids. Biochem Pharmacol. 1971 Oct;20(10):2797–2804. doi: 10.1016/0006-2952(71)90190-0. [DOI] [PubMed] [Google Scholar]
- Cutroneo K. R., Counts D. F. Anti-inflammatory steroids and collagen metabolism: glucocorticoid-mediated alterations of prolyl hydroxylase activity and collagen synthesis. Mol Pharmacol. 1975 Sep;11(5):632–639. [PubMed] [Google Scholar]
- Freeman P. C., Mangan F. R., Watkins D. K. Some effects of hydrocortisone on the early development of the rat cotton pellet granuloma. Biochem Pharmacol. 1979 Mar 1;28(5):573–578. doi: 10.1016/0006-2952(79)90137-0. [DOI] [PubMed] [Google Scholar]
- Kulonen E., Potila M. Effect of the administration of antirheumatic drugs on experimental granuloma in rat. Biochem Pharmacol. 1975 Jan 15;24(2):219–225. doi: 10.1016/0006-2952(75)90280-4. [DOI] [PubMed] [Google Scholar]
- Nakagawa H., Ohkoshi K., Tsurufuji S. Effect of D-penicillamine and beta-aminopropionitrile on collagen breakdown of carrageenin granuloma in rats. Biochem Pharmacol. 1976 May 15;25(10):1129–1132. doi: 10.1016/0006-2952(76)90358-0. [DOI] [PubMed] [Google Scholar]
- Nimni M. E., Deshmukh K., Gerth N., Bavetta L. A. Changes in collagen metabolism associated with the administration of penicillamine and various amino and thiol compounds. Biochem Pharmacol. 1969 Apr;18(4):707–714. doi: 10.1016/0006-2952(69)90041-0. [DOI] [PubMed] [Google Scholar]
- Nimni M. E., Deshmukh K., Gerth N. Collagen defect induced by penicillamine. Nat New Biol. 1972 Dec 13;240(102):220–221. doi: 10.1038/newbio240220a0. [DOI] [PubMed] [Google Scholar]
- Nimni M. E. Mechanism of inhibition of collagen crosslinking by penicillamine. Proc R Soc Med. 1977;70 (Suppl 3):65–72. doi: 10.1177/00359157770700S322. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oikarinen A. Effect of betamethasone-17-valerate on synthesis of collagen and prolyl hydroxylase activity in chick embryo tendon cells. Biochem Pharmacol. 1977 May 1;26(9):875–879. doi: 10.1016/0006-2952(77)90403-8. [DOI] [PubMed] [Google Scholar]
- WOESSNER J. F., Jr The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys. 1961 May;93:440–447. doi: 10.1016/0003-9861(61)90291-0. [DOI] [PubMed] [Google Scholar]