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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1981 Jun;62(3):308–316.

Antigen-induced and zymosan-induced arthritis in mice: studies on in vivo cartilage proteoglycan synthesis and chondrocyte death.

W B van den Berg, M W Kruijsen, L B van de Putte, H J van Beusekom, M van der Sluis-van der Pol, W A Zwarts
PMCID: PMC2041692  PMID: 7248172

Abstract

The influence of joint inflammation on patellar hyaline articular cartilage was studied in mice. Antigen-induced and zymosan-induced arthritis were used as models for immunologically and non-immunologically induced joint inflammation. The contribution of newly formed proteoglycan to the cartilage proteoglycan content, as measured by labelling of the cartilage after i.v. administration of 35S-sulphate, was decreased in parallel with the severity of inflammation during both zymosan-induced and antigen-induced arthritis. The decreased 35S content of the cartilage was due to inhibition of synthesis rather than breakdown of newly synthesized proteoglycan, since no accelerated release of 35S from arthritic cartilage could be demonstrated in vitro. Antigen-induced arthritis was associated with progressive chondrocyte damage. Loss of chondrocytes was consistently found in the central part of the patella, without nearby presence of pannus. It would appear that, in addition to enzymatic breakdown of cartilage, other phenomena are important in cartilage destruction: inhibition of proteoglycan synthesis and chondrocyte death, apparently unrelated to pannus formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brackertz D., Mitchell G. F., Mackay I. R. Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum. 1977 Apr;20(3):841–850. doi: 10.1002/art.1780200314. [DOI] [PubMed] [Google Scholar]
  2. Carmichael D. J., Gillard G. C., Lowther D. A., Handley C. J., Santer V. B. Carrageenin-induced arthritis. IV. Rate changes in cartilage matrix proteoglycan synthesis. Arthritis Rheum. 1977 Apr;20(3):834–840. doi: 10.1002/art.1780200313. [DOI] [PubMed] [Google Scholar]
  3. Dingle J. T. Heberden oration 1978. Recent studies on the control of joint damage: the contribution of the Strangeways Research Laboratory. Ann Rheum Dis. 1979 Jun;38(3):201–214. doi: 10.1136/ard.38.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Handley C. J., Lowther D. A. Inhibition of proteoglycan biosynthesis by hyaluronic acid in chondrocytes in cell culture. Biochim Biophys Acta. 1976 Aug 24;444(1):69–74. doi: 10.1016/0304-4165(76)90224-5. [DOI] [PubMed] [Google Scholar]
  5. Janis R., Hamerman D. Articular cartilage changes in early arthritis. Bull Hosp Joint Dis. 1969 Oct;30(2):136–152. [PubMed] [Google Scholar]
  6. Jubb R. W., Fell H. B. The effect of synovial tissue on the synthesis of proteoglycan by the articular cartilage of young pigs. Arthritis Rheum. 1980 May;23(5):545–555. doi: 10.1002/art.1780230505. [DOI] [PubMed] [Google Scholar]
  7. Keystone E. C., Schorlemmer H. U., Pope C., Allison A. C. Zymosan-induced arthritis: a model of chronic proliferative arthritis following activation of the alternative pathway of complement. Arthritis Rheum. 1977 Sep-Oct;20(7):1396–1401. doi: 10.1002/art.1780200714. [DOI] [PubMed] [Google Scholar]
  8. Lowther D. A., Gillard G. C. Carrageenin-induced arthritis. I. The effect of intraarticular carrageenin on the chemical composition of articular cartilage. Arthritis Rheum. 1976 Jul-Aug;19(4):769–776. doi: 10.1002/1529-0131(197607/08)19:4<769::aid-art1780190419>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  9. Lowther D. A., Sandy J. D., Santer V. B., Brown H. L. Antigen-induced arthritis. Decreased proteoglycan content and inhibition of proteoglycan synthesis in articular cartilage. Arthritis Rheum. 1978 Jul-Aug;21(6):675–680. doi: 10.1002/art.1780210611. [DOI] [PubMed] [Google Scholar]
  10. Ohno O., Tateishi H., Cooke T. D. Pathogenesis of chronic inflammation in experimental ferritin-induced arthritis. IV. Immuno-electron microscopic techniques in the study of articular collagenous tissues. Arthritis Rheum. 1978 Jan-Feb;21(1):81–91. doi: 10.1002/art.1780210114. [DOI] [PubMed] [Google Scholar]
  11. Sandy J. D., Lowther D. A., Brown H. L. Antigen-induced arthritis. Studies on the inhibition of proteoglycan synthesis observed in articular cartilage during short-term joint inflammation. Arthritis Rheum. 1980 Apr;23(4):433–447. doi: 10.1002/art.1780230406. [DOI] [PubMed] [Google Scholar]
  12. Stastny P., Cooke T. D., Ziff M. Production of a macrophage migration inhibitory factor in rabbits with experimental arthritis. Clin Exp Immunol. 1973 May;14(1):141–147. [PMC free article] [PubMed] [Google Scholar]
  13. Stastny P., Rosenthal M., Andreis M., Cooke D., Ziff M. Lymphokines in rheumatoid synovitis. Ann N Y Acad Sci. 1975 Jun 13;256:117–131. doi: 10.1111/j.1749-6632.1975.tb36041.x. [DOI] [PubMed] [Google Scholar]

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