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. 1987 Nov;53(11):2666–2670. doi: 10.1128/aem.53.11.2666-2670.1987

Methods for recovering poliovirus and rotavirus from oysters.

J I Speirs 1, R D Pontefract 1, J Harwig 1
PMCID: PMC204170  PMID: 2827573

Abstract

Polioviruses and rotaviruses are potential indicators of sewage pollution of water and shellfish. Several methods for detecting these viruses in oysters were assessed. Elution-precipitation involving Catfloc for clarification and skim milk for subsequent flocculation resulted in the recovery of an average of 79% of poliovirus type 1 and 37% of rotavirus SA-11 from oyster homogenates inoculated with low numbers of these viruses. Adsorption-elution-precipitation did not improve the recovery of poliovirus and was detrimental to the recovery of rotavirus. Ultrafiltration or ultracentrifugation resulted in improved recovery of rotavirus but also in higher toxicity of oyster extracts to cell cultures. We recommend the use of the described elution-precipitation method for detecting viral pollutants in sample of oysters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Goyal S. M., Gerba C. P., Melnick J. L. Human enteroviruses in oysters and their overlying waters. Appl Environ Microbiol. 1979 Mar;37(3):572–581. doi: 10.1128/aem.37.3.572-581.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harris J. R., Cohen M. L., Lippy E. C. Water-related disease outbreaks in the United States, 1981. J Infect Dis. 1983 Oct;148(4):759–762. doi: 10.1093/infdis/148.4.759. [DOI] [PubMed] [Google Scholar]
  3. Hurst C. J., Dahling D. R., Safferman R. S., Goyke T. Comparison of commercial beef extracts and similar materials for recovering viruses from environmental samples. Can J Microbiol. 1984 Oct;30(10):1253–1263. doi: 10.1139/m84-198. [DOI] [PubMed] [Google Scholar]
  4. Johnson K. M., Cooper R. C., Straube D. C. Procedure for recovery of enteroviruses from the Japanese cockle Tapes japonica. Appl Environ Microbiol. 1981 Apr;41(4):932–935. doi: 10.1128/aem.41.4.932-935.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Katzenelson E., Fattal B., Hostovesky T. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water. Appl Environ Microbiol. 1976 Oct;32(4):638–639. doi: 10.1128/aem.32.4.638-639.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Konowalchuk J., Speirs J. I. Enterovirus recovery from laboratory-contaminated samples of shellfish. Can J Microbiol. 1972 Jul;18(7):1023–1029. doi: 10.1139/m72-159. [DOI] [PubMed] [Google Scholar]
  7. Kostenbader K. D., Jr, Cliver D. O. Polyelectrolyte flocculation as an aid to recovery of enteroviruses from oysters. Appl Microbiol. 1972 Oct;24(4):540–543. doi: 10.1128/am.24.4.540-543.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Metcalf T. G., Moulton E., Eckerson D. Improved method and test strategy for recovery of enteric viruses from shellfish. Appl Environ Microbiol. 1980 Jan;39(1):141–152. doi: 10.1128/aem.39.1.141-152.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ramia S., Sattar S. A. Concentration of seeded simian rotavirus SA-11 from potable waters by using talc-celite layers and hydroextraction. Appl Environ Microbiol. 1980 Mar;39(3):493–499. doi: 10.1128/aem.39.3.493-499.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rao V. C., Seidel K. M., Goyal S. M., Metcalf T. G., Melnick J. L. Isolation of enteroviruses from water, suspended solids, and sediments from Galveston Bay: survival of poliovirus and rotavirus adsorbed to sediments. Appl Environ Microbiol. 1984 Aug;48(2):404–409. doi: 10.1128/aem.48.2.404-409.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richards G. P., Goldmintz D., Green D. L., Babinchak J. A. Rapid methods for extraction and concentration of poliovirus from oyster tissues. J Virol Methods. 1982 Dec;5(5-6):285–291. doi: 10.1016/0166-0934(82)90019-2. [DOI] [PubMed] [Google Scholar]
  12. Seidel K. M., Goyal S. M., Rao V. C., Melnick J. L. Concentration of rotavirus and enteroviruses from blue crabs (Callinectes sapidus). Appl Environ Microbiol. 1983 Dec;46(6):1293–1296. doi: 10.1128/aem.46.6.1293-1296.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith E. M., Gerba C. P. Development of a method for detection of human rotavirus in water and sewage. Appl Environ Microbiol. 1982 Jun;43(6):1440–1450. doi: 10.1128/aem.43.6.1440-1450.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sobsey M. D., Carrick R. J., Jensen H. R. Improved methods for detecting enteric viruses in oysters. Appl Environ Microbiol. 1978 Jul;36(1):121–128. doi: 10.1128/aem.36.1.121-128.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steinmann J. Detection of rotavirus in sewage. Appl Environ Microbiol. 1981 Apr;41(4):1043–1045. doi: 10.1128/aem.41.4.1043-1045.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tierney J. T., Sullivan R., Peeler J. T., Larkin E. P. Detection of low numbers of poliovirus 1 in oysters: collaborative study. J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):884–886. [PubMed] [Google Scholar]
  17. Vaughn J. M., Landry E. F., Vicale T. J., Dahl M. C. Modified procedure for the recovery of naturally accumulated poliovirus from oysters. Appl Environ Microbiol. 1979 Oct;38(4):594–598. doi: 10.1128/aem.38.4.594-598.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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