Abstract
Nine different inbred strains of mice inoculated intraperitoneally with yeast cells of Paracoccidioides brasiliensis showed significantly varying patterns of susceptibility. The A/SN strain was found to be the most resistant, while BIOD2/nSn, BIO.A and BIOD2/oSn the most susceptible strains. These susceptibility differences were not dependent on the size of challenge inocula and sex of animals. All strains studied showed a mean survival time proportional to the size of inocula used. Although almost all infected male mice presented a shorter survival time when compared with females, significant mortality differences between sexes were found only in two of the strains studied, namely BALB/c and BIOD2/nSn. The H-2 region did not influence the susceptibility pattern since the A/SN and BIO.A strains share the same H-2 haplotype and were respectively highly resistant and susceptible to P. brasiliensis. Furthermore, the presence of C5 and unresponsiveness to lipopolysaccharide had no influence on the mortality data observed. Specific antibodies were detected only in a small number of animals and titres were consistently low, appearing later in the resistant (A/SN) than in a susceptible strain (BIO.A). Omentum, spleen and liver were the most affected organs in both strains, but the susceptible mice had more granulomatous lesions and earlier dissemination of the fungus.
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- BUSSARD A. [Description of a technic simultaneously combining electrophoresis and immunological precipitation in gel: electrosyneresis]. Biochim Biophys Acta. 1959 Jul;34:258–260. doi: 10.1016/0006-3002(59)90261-6. [DOI] [PubMed] [Google Scholar]
- Berliner M. D., Reca M. E. Vital staining of Histoplasma capsulatum with Janus Green B. Sabouraudia. 1966 Jun;5(1):26–29. [PubMed] [Google Scholar]
- Chick E. W., Roberts G. D. The varying susceptibility of different genetic strains of laboratory mice to Histoplasma capsulatum. Mycopathol Mycol Appl. 1974 Apr 30;52(3):251–253. doi: 10.1007/BF02198750. [DOI] [PubMed] [Google Scholar]
- Londero A. T., Ramos C. D. Paracoccidioidomycosis. A clinical and mycologic study of forty-one cases observed in Santa Maria, RS, Brazil. Am J Med. 1972 Jun;52(6):771–775. doi: 10.1016/0002-9343(72)90083-6. [DOI] [PubMed] [Google Scholar]
- Moscardi M., Franco M. F. Paracoccidioidomicose experimental do camundongo. I--Aspectos immunopatológicos da infecço intraperitoneal. Rev Inst Med Trop Sao Paulo. 1980 Nov-Dec;22(6):286–293. [PubMed] [Google Scholar]
- Musatti C. C., Rezkallah M. T., Mendes E., Mendes N. F. In vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidiodomycosis. Cell Immunol. 1976 Jun 15;24(2):365–378. doi: 10.1016/0008-8749(76)90220-3. [DOI] [PubMed] [Google Scholar]
- Netto C. F., Castro R. M., Gonçalves A. P., Dillon N. L. Ocorrência familiar da blastomicose sul-americana. A propósito de 14 casos. Rev Inst Med Trop Sao Paulo. 1965 Nov-Dec;7(6):332–336. [PubMed] [Google Scholar]
- Rhodes J. C., Wicker L. S., Urba W. J. Genetic control of susceptibility to Cryptococcus neoformans in mice. Infect Immun. 1980 Aug;29(2):494–499. doi: 10.1128/iai.29.2.494-499.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruco L. P., Meltzer M. S. Defective tumoricidal capacity of macrophages from C3H/HeJ mice. J Immunol. 1978 Jan;120(1):329–334. [PubMed] [Google Scholar]
- Ruco L. P., Meltzer M. S., Rosenstreich D. L. Macrophage activation for tumor cytotoxicity: control of macrophage tumoricidal capacity by the LPS gene. J Immunol. 1978 Aug;121(2):543–548. [PubMed] [Google Scholar]