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. 1987 Dec;53(12):2862–2865. doi: 10.1128/aem.53.12.2862-2865.1987

Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count.

P R Brayton 1, M L Tamplin 1, A Huq 1, R R Colwell 1
PMCID: PMC204213  PMID: 3324967

Abstract

A field trial to enumerate Vibrio cholerae O1 in aquatic environments in Bangladesh was conducted, comparing fluorescent-antibody direct viable count with culture detection by the most-probable-number index. Specificity of a monoclonal antibody prepared against the O1 antigen was assessed and incorporated into the fluorescence staining method. All pond and water samples yielded higher counts of viable V. cholerae O1 by fluorescent-antibody direct viable count than by the most-probable-number index. Fluorescence microscopy is a more sensitive detection system than culture methods because it allows the enumeration of both culturable and nonculturable cells and therefore provides more precise monitoring of microbiological water quality.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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