Abstract
Cellular fatty acids and monosaccharides in a group of 14 lactobacilli were analyzed by gas chromatography and the identity of the components was confirmed by gas chromatography-mass spectrometry. From the same bacterial sample, both monosaccharides and fatty acids were liberated by methanolysis, and in certain experiments, fatty acids alone were released by basic hydrolysis. The results indicate that basic hydrolysis gave more comprehensive information about the fatty acids, but the analysis of monosaccharides was found to be much more useful in distinguishing between different species of lactobacilli. The method described allowed differentiation of 11 of 14 Lactobacillus species, and even single colonies isolated from agar plates could be used for analysis without subculturing.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chambers R. E., Clamp J. R. An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials. Biochem J. 1971 Dec;125(4):1009–1018. doi: 10.1042/bj1251009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clay K. L., Murphy R. C. New procedure for isolation of amino acids based on selective hydrolysis of trimethylsilyl derivatives. J Chromatogr. 1979 Dec 1;164(4):417–426. doi: 10.1016/s0378-4347(00)81545-5. [DOI] [PubMed] [Google Scholar]
- Edwards C., McCann R. J. The effects of nalidixic acid on respiratory activity of asynchronous and synchronous cultures of Alcaligenes eutrophus. J Gen Microbiol. 1983 Jan;129(1):1–5. doi: 10.1099/00221287-129-1-1. [DOI] [PubMed] [Google Scholar]
- Farshtchi D., McClung N. M. Effect of substrate on fatty acid production in Nocardia asteroides. Can J Microbiol. 1970 Apr;16(4):213–217. doi: 10.1139/m70-039. [DOI] [PubMed] [Google Scholar]
- Knivett V. A., Cullen J. Some factors affecting cyclopropane acid formation in Escherichia coli. Biochem J. 1965 Sep;96(3):771–776. doi: 10.1042/bj0960771. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lambert M. A., Moss C. W. Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. J Clin Microbiol. 1983 Dec;18(6):1370–1377. doi: 10.1128/jcm.18.6.1370-1377.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lambert M. S., Moss C. W. Degradation of bacterial cyclopropane acids with boron trihalide reagents. Microbios. 1977;18(71):51–58. [PubMed] [Google Scholar]
- Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rizzo A. F. Rapid gas-chromatographic method for identification of metabolic products of anaerobic bacteria. J Clin Microbiol. 1980 Apr;11(4):418–421. doi: 10.1128/jcm.11.4.418-421.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schneitz C., Seuna E., Rizzo A. The anaerobically cultured cecal flora of adult fowls that protects chickens from Salmonella infections. Acta Pathol Microbiol Scand B. 1981 Apr;89(2):109–116. doi: 10.1111/j.1699-0463.1981.tb00161_89b.x. [DOI] [PubMed] [Google Scholar]
- Veerkamp J. H. Fatty acid composition of Bifidobacterium and Lactobacillus strains. J Bacteriol. 1971 Nov;108(2):861–867. doi: 10.1128/jb.108.2.861-867.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Váczi L., Rédai I., Réthy A. Changes in the fatty acid composition of Staphylococcus aureus under various cultural conditions. Acta Microbiol Acad Sci Hung. 1967;14(3):293–298. [PubMed] [Google Scholar]