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. 1987 Dec;53(12):2967–2971. doi: 10.1128/aem.53.12.2967-2971.1987

Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.

A M Nasser 1, T G Metcalf 1
PMCID: PMC204231  PMID: 2829721

Abstract

Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.

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Selected References

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  1. Anderson D. A. Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175. J Med Virol. 1987 May;22(1):35–44. doi: 10.1002/jmv.1890220106. [DOI] [PubMed] [Google Scholar]
  2. Cromeans T., Sobsey M. D., Fields H. A. Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus. J Med Virol. 1987 May;22(1):45–56. doi: 10.1002/jmv.1890220107. [DOI] [PubMed] [Google Scholar]
  3. Daemer R. J., Feinstone S. M., Gust I. D., Purcell R. H. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect Immun. 1981 Apr;32(1):388–393. doi: 10.1128/iai.32.1.388-393.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  5. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol. 1982 Sep;43(3):997–1005. doi: 10.1128/jvi.43.3.997-1005.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flehmig B. Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Med Microbiol Immunol. 1980;168(4):239–248. doi: 10.1007/BF02121807. [DOI] [PubMed] [Google Scholar]
  7. Frösner G. G., Deinhardt F., Scheid R., Gauss-Müller V., Holmes N., Messelberger V., Siegl G., Alexander J. J. Propagation of human hepatitis A virus in a hepatoma cell line. Infection. 1979;7(6):303–305. doi: 10.1007/BF01642154. [DOI] [PubMed] [Google Scholar]
  8. Gauss-Müller V., Frösner G. G., Deinhardt F. Propagation of hepatitis A virus in human embryo fibroblasts. J Med Virol. 1981;7(3):233–239. doi: 10.1002/jmv.1890070308. [DOI] [PubMed] [Google Scholar]
  9. Hollinger F. B., Bradley D. W., Maynard J. E., Dreesman G. R., Melnick J. L. Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975 Nov;115(5):1464–1466. [PubMed] [Google Scholar]
  10. Jiang X., Estes M. K., Metcalf T. G., Melnick J. L. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes. Appl Environ Microbiol. 1986 Oct;52(4):711–717. doi: 10.1128/aem.52.4.711-717.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lemon S. M., Binn L. N., Marchwicki R. H. Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. J Clin Microbiol. 1983 May;17(5):834–839. doi: 10.1128/jcm.17.5.834-839.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nasser A. M., Metcalf T. G. An A-ELISA to detect hepatitis A virus in estuarine samples. Appl Environ Microbiol. 1987 May;53(5):1192–1195. doi: 10.1128/aem.53.5.1192-1195.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Parry J. V., Richmond J. E., Gardner S. D. Polyomavirus in fetal rhesus monkey kidney cell lines used to grow hepatitis A virus. Lancet. 1983 Apr 30;1(8331):994–994. doi: 10.1016/s0140-6736(83)92121-9. [DOI] [PubMed] [Google Scholar]
  14. Plagemann P. G., Swim H. E. Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. J Bacteriol. 1966 Jun;91(6):2317–2326. doi: 10.1128/jb.91.6.2317-2326.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Provost P. J., Hilleman M. R. Propagation of human hepatitis A virus in cell culture in vitro. Proc Soc Exp Biol Med. 1979 Feb;160(2):213–221. doi: 10.3181/00379727-160-40422. [DOI] [PubMed] [Google Scholar]
  16. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  17. SMITH K. O., MELNICK J. L. Electron microscopic counting of virus particles by sedimentation on aluminized grids. J Immunol. 1962 Aug;89:279–284. [PubMed] [Google Scholar]
  18. Salacinski P. R., McLean C., Sykes J. E., Clement-Jones V. V., Lowry P. J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981 Oct;117(1):136–146. doi: 10.1016/0003-2697(81)90703-x. [DOI] [PubMed] [Google Scholar]
  19. Simmonds R. S., Szücs G., Metcalf T. G., Melnick J. L. Persistently infected cultures as a source of hepatitis A virus. Appl Environ Microbiol. 1985 Apr;49(4):749–755. doi: 10.1128/aem.49.4.749-755.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Venuti A., Di Russo C., del Grosso N., Patti A. M., Ruggeri F., De Stasio P. R., Martiniello M. G., Pagnotti P., Degener A. M., Midulla M. Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form. J Virol. 1985 Nov;56(2):579–588. doi: 10.1128/jvi.56.2.579-588.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wheeler C. M., Fields H. A., Schable C. A., Meinke W. J., Maynard J. E. Adsorption, purification, and growth characteristics of hepatitis A virus strain HAS-15 propagated in fetal rhesus monkey kidney cells. J Clin Microbiol. 1986 Mar;23(3):434–440. doi: 10.1128/jcm.23.3.434-440.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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