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. 1987 Dec;53(12):2975–2977. doi: 10.1128/aem.53.12.2975-2977.1987

Cloning of the Pachysolen tannophilus Xylulokinase Gene by Complementation in Escherichia coli

Panayiotis E Stevis 1, James J Huang 1,†, Nancy W Y Ho 1,*
PMCID: PMC204233  PMID: 16347513

Abstract

The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Saccharomyces cerevisiae xylulokinase genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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