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British Journal of Clinical Pharmacology logoLink to British Journal of Clinical Pharmacology
. 1997 Mar;43(3):329–332. doi: 10.1046/j.1365-2125.1997.00545.x

The effect of the neutral endopeptidase inhibitor drug, candoxatril, on circulating levels of two of the most potent vasoactive peptides

G McDowell 1, W Coutie 2, C Shaw 1, K D Buchanan 1, A D Struthers 2, D P Nicholls 1
PMCID: PMC2042739  PMID: 9088591

Abstract

Aims Candoxatril is a specific neutral endopeptidase (NEP) 24.11 inhibitor, and previous work has documented the effect of NEP inhibition on atrial and brain natriuretic peptides (ANP, BNP). The aim of the present study was to ascertain the effect of NEP inhibition on circulating levels of vasoactive peptides.

Methods We studied seven patients with chronic heart failure who were randomized to receive candoxatril, candoxatril/captopril, captopril and placebo in a four way cross over, double-blind pattern.

Results The mean circulating level of endothelin (ET) was increased 2 h after placebo (10 to 20 pg ml−1 ) and also calcitonin gene-related peptide (CGRP) (27 to 51 pg ml−1 ), but ANP levels changed little during this time (73 to 75 pg ml−1 ). Following candoxatril, however, ET (10 to 39 pg ml−1, P<0.05), CGRP (34 to 99 pg ml−1, P<0.05) and ANP (72 to 108 pg ml−1, P<0.05) increased further. A similar result was observed following combined treatment with candoxatril and captopril.

Conclusions We conclude that neutral endopeptidase inhibition increases circulating plasma levels of ET and CGRP in addition to the natriuretic peptides.

Keywords: candoxatril, neutral endopeptidase inhibitor, brain natriuretic peptide, atrial natriuretic peptide

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