Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Oct;54(10):2452–2459. doi: 10.1128/aem.54.10.2452-2459.1988

Influence of readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp.

E Topp 1, R L Crawford 1, R S Hanson 1
PMCID: PMC204284  PMID: 3202628

Abstract

The influence of high concentrations of pentachlorophenol (PCP) and readily metabolizable carbon on the activity and viability of a PCP-degrading Flavobacterium sp. was examined in a mineral salts medium. Lags preceding PCP removal by glutamate-grown Flavobacterium cells were greatly attenuated by the addition of glutamate, aspartate, succinate, acetate, glucose, or cellobiose. The effect of these supplementary carbon sources on the apparent lag was not mediated entirely through the stimulation of growth since PCP metabolism accompanied the onset of growth. The specific activity of PCP-degrading cells in the absence of supplementary carbon was 1.51 x 10(-13) +/- 0.08 x 10(-13) g of PCP per cell per h and in the presence of supplementary carbon was 0.92 x 10(-13) +/- 0.09 x 10(-13) g of PCP per cell per h. Glutamate in combination with glucose or cellobiose partially repressed PCP metabolism. PCP removal by PCP-induced, glutamate-grown cells suspended in the presence of 4 g of sodium glutamate per liter was sensitive to shock loads of PCP, with a Ki of about 86.8 micrograms/ml. Subsequent removal rates, however, were more resistant to PCP. Optimal stimulation of PCP removal by sodium glutamate required 3.0 g/liter, about the same concentration as that which saturated growth in the absence of PCP. PCP removal rates decayed within minutes following the transfer of PCP-induced, glutamate-grown cells to media containing PCP without supplementary carbon, and increasing PCP concentrations accelerated the decay.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
2452

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apajalahti J. H., Salkinoja-Salonen M. S. Complete dechlorination of tetrachlorohydroquinone by cell extracts of pentachlorophenol-induced Rhodococcus chlorophenolicus. J Bacteriol. 1987 Nov;169(11):5125–5130. doi: 10.1128/jb.169.11.5125-5130.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown M. R., Williams P. Influence of substrate limitation and growth phase on sensitivity to antimicrobial agents. J Antimicrob Chemother. 1985 Jan;15 (Suppl A):7–14. doi: 10.1093/jac/15.suppl_a.7. [DOI] [PubMed] [Google Scholar]
  3. Chu J. P., Kirsch E. J. Metabolism of pentachlorophenol by an axenic bacterial culture. Appl Microbiol. 1972 May;23(5):1033–1035. doi: 10.1128/am.23.5.1033-1035.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dall-Larsen T., Kryvi H., Klungsoyr L. Dinitrophenol, dicoumarol and pentachlorophenol as inhibitors and parasite substrates in the ATP phosphoribosyltransferase reaction. Eur J Biochem. 1976 Jul 15;66(3):443–446. doi: 10.1111/j.1432-1033.1976.tb10568.x. [DOI] [PubMed] [Google Scholar]
  5. Duxbury C. L., Thompson J. E. Pentachlorophenol alters the molecular organization of membranes in mammalian cells. Arch Environ Contam Toxicol. 1987 May;16(3):367–373. doi: 10.1007/BF01054955. [DOI] [PubMed] [Google Scholar]
  6. Edgehill R. U., Finn R. K. Microbial treatment of soil to remove pentachlorophenol. Appl Environ Microbiol. 1983 Mar;45(3):1122–1125. doi: 10.1128/aem.45.3.1122-1125.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Flechtner V. R., Hanson R. S. Coarse and fine control of citrate synthase from Bacillus subtilis. Biochim Biophys Acta. 1969 Jul 30;184(2):252–262. doi: 10.1016/0304-4165(69)90027-0. [DOI] [PubMed] [Google Scholar]
  8. Gilbert P., Brown M. R. Influence of growth rate and nutrient limitation on the gross cellular composition of Pseudomonas aeruginosa and its resistance to 3- and 4-chlorophenol. J Bacteriol. 1978 Mar;133(3):1066–1072. doi: 10.1128/jb.133.3.1066-1072.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harder W., Dijkhuizen L. Strategies of mixed substrate utilization in microorganisms. Philos Trans R Soc Lond B Biol Sci. 1982 Jun 11;297(1088):459–480. doi: 10.1098/rstb.1982.0055. [DOI] [PubMed] [Google Scholar]
  10. Kilbane J. J., Chatterjee D. K., Chakrabarty A. M. Detoxification of 2,4,5-trichlorophenoxyacetic acid from contaminated soil by Pseudomonas cepacia. Appl Environ Microbiol. 1983 May;45(5):1697–1700. doi: 10.1128/aem.45.5.1697-1700.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pignatello J. J., Martinson M. M., Steiert J. G., Carlson R. E., Crawford R. L. Biodegradation and photolysis of pentachlorophenol in artificial freshwater streams. Appl Environ Microbiol. 1983 Nov;46(5):1024–1031. doi: 10.1128/aem.46.5.1024-1031.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saber D. L., Crawford R. L. Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol. Appl Environ Microbiol. 1985 Dec;50(6):1512–1518. doi: 10.1128/aem.50.6.1512-1518.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stanlake G. J., Finn R. K. Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1982 Dec;44(6):1421–1427. doi: 10.1128/aem.44.6.1421-1427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steiert J. G., Crawford R. L. Catabolism of pentachlorophenol by a Flavobacterium sp. Biochem Biophys Res Commun. 1986 Dec 15;141(2):825–830. doi: 10.1016/s0006-291x(86)80247-9. [DOI] [PubMed] [Google Scholar]
  15. Steiert J. G., Pignatello J. J., Crawford R. L. Degradation of chlorinated phenols by a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1987 May;53(5):907–910. doi: 10.1128/aem.53.5.907-910.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stockdale M., Selwyn M. J. Influence of ring substituents on the action of phenols on some dehydrogenases, phospholinases and the soluble ATPase from mitochondria. Eur J Biochem. 1971 Aug 16;21(3):416–423. doi: 10.1111/j.1432-1033.1971.tb01486.x. [DOI] [PubMed] [Google Scholar]
  17. Suzuki T. Metabolism of pentachlorophenol by a soil microbe. J Environ Sci Health B. 1977;12(2):113–127. doi: 10.1080/03601237709372057. [DOI] [PubMed] [Google Scholar]
  18. WEINBACH E. C., GARBUS J. THE INTERACTION OF UNCOUPLING PHENOLS WITH MITOCHONDRIA AND WITH MITOCHONDRIAL PROTEIN. J Biol Chem. 1965 Apr;240:1811–1819. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES