Abstract
Nitric oxide (NO) is an efficient antimicrobial agent. A role for mycothiol in protecting mycobacteria from nitrosative damage was revealed by showing that a Mycobacterium smegmatis mutant is sensitive to NO. A direct correlation between NO and mycothiol levels confirmed that mycothiol is important for protecting mycobacteria from NO attack.
Mycobacteria encounter free radicals, such as reactive oxygen and nitrogen intermediates (ROIs and RNIs), as a result of normal aerobic respiration and during infection as part of host defenses. An immediate response to free radicals is provided by the thiol analog of glutathione, mycothiol (MSH). MSH also maintains a stable intracellular redox environment (3, 18) and detoxifies toxic substances (11, 15, 18). Xenobiotic detoxification is achieved by three different thiol-dependent means: (i) donation of electrons by thiols to peroxidases that catalyze the conversion of peroxides to alcohols or water, (ii) reduction of substrates in a reaction coupled with thiol oxidation (18), and (iii) conjugation of thiols to electrophiles, such as antibiotics, and production of less toxic mercapturic acids (11, 15). More importantly, an MSNO (S-nitrosomycothiol)-dependent nitrosomycothiol reductase has been characterized (19).
We have shown that exogenous gaseous nitric oxide (gNO) is able to eradicate a variety of bacterial species, including clinical isolates (4, 5, 10). In this study, we examined the abilities of mycobacteria to resist killing during gNO treatment and the role of MSH in protection against RNIs.
Exogenous nitric oxide exerts an antimicrobial effect on Mycobacterium smegmatis.
The antimicrobial action of gNO on M. smegmatis mc2155 was assessed by exposing approximately 105 CFU/ml to 200 ppm gNO, using our previously described apparatus and methodology (5), and determining bacterial viability. Over time, continuous exposure decreased the concentration of viable bacteria (Fig. 1A). The sharp decrease in bacterial viability followed a 7-hour latency period, and by 10 h, no viable bacteria could be recovered from the suspension. In contrast, in the control sample, the number of viable bacteria remained relatively stable, with limited decrease in viability over a period of 11 h.
FIG. 1.
Antimicrobial activity of gNO on M. smegmatis mc2155 in 0.9% saline solution. Mycobacteria were exposed to 200 ppm gNO (mc2155, open squares; mshA::Tn5, open circles; mshA::Tn5 [pAL0486], open triangles) or medical air (mc2155, filled squares; mshA::Tn5, filled circles; mshA::Tn5 [pAL0486], filled triangles). (A) Killing pattern of M. smegmatis. (B) Increased sensitivity to gNO-mediated killing for MSH-deficient M. smegmatis mshA::Tn5 compared to that for its wild-type parent. (C) Complementing M. smegmatis mshA::Tn5 with cloned M. tuberculosis mshA expressed on a plasmid restored the parental level of resistance to gNO-mediated killing. In all cases, to avoid the effect of gNO on growth media compounds, bacteria were suspended in 0.9% saline. Each data point represents the mean ± standard deviation for at least three independent measurements.
Mycobacteria are more resistant to gNO than other bacteria.
M. smegmatis had the longest latency period (defined as the time required for a 10-fold decrease in CFU) of any of the bacteria tested (Table 1) and the highest 100% lethal dose (LD100) at 200 ppm h−1 gNO. As a result, M. smegmatis required the highest dose of gNO (2,000 ppm-h) to achieve complete killing in suspension.
TABLE 1.
Killing of bacterial isolates exposed continuously to 200 ppm gNO
Bacterium | Latency period (h)a | LD100 (h)b | Dose (ppm-h)c |
---|---|---|---|
S. aureus | 3 | 4 | 800 |
Pseudomonas aeruginosa | 1 | 3 | 600 |
Staphylococcus aureus MRSA | 3 | 5 | 1,000 |
Serratia sp. | 4 | 6 | 1,200 |
Klebsiella sp. no. 1 | 3 | 6 | 1,200 |
Klebsiella sp. no. 2 | 2 | 5 | 1,000 |
Klebsiella sp. no. 3 | 3 | 6 | 1,200 |
Enterobacter sp. | 5 | 6 | 1,200 |
Acinetobacter sp. | 5 | 6 | 1,200 |
Candida albicans | 2 | 4 | 800 |
M. smegmatis | 7 | 10 | 2,000 |
Escherichia coli | 3 | 5 | 1,000 |
Group B Streptococcus | 1 | 2 | 400 |
Latency period was defined as the exposure time required for viability to decrease 10-fold.
LD100 was defined as the exposure time at which viable bacteria could no longer be recovered.
Dose was defined as the product of the LD100 and the gNO concentration to which the bacteria were exposed.
The M. smegmatis mshA mutant is more sensitive to gNO.
Mycobacteria are known to possess detoxification systems that offer protection from the toxic effects of ROIs and RNIs (1, 8, 9, 11, 13, 14, 17). However, the fact that there is a delay in gNO effect that could eventually be overcome with continuous exposure to gNO suggests that a cellular component that could be depleted is involved. To determine if MSH plays a direct role in protecting mycobacteria from gNO, an M. smegmatis mshA::Tn5 (A1) (12) mutant unable to produce MSH was exposed to gNO. The latency period for the mutant was reduced to 4 h, compared to 7 h for the wild-type parent (Fig. 1B).
To confirm that the shortened latency period observed for M. smegmatis mshA::Tn5 was due only to the missing MSH biosynthetic activity encoded by mshA, the Mycobacterium tuberculosis mshA gene was supplied in trans on the plasmid pAL0486 and the complemented strain was tested. The latency period for mshA::Tn5 exposed to gNO was again shorter than that for the wild-type parent strain (4 h versus 6 h), but in the complemented strain, the latency period increased to 6 h, similar to that for the wild-type parent (Fig. 1C). The basal MSH levels in the mutant, the complemented strain, and wild-type M. smegmatis agreed with the levels previously measured by Newton et al. (12).
MSH levels decrease on exposure to nitric oxide.
To determine if MSH levels were affected by gNO, M. smegmatis mc2155 was exposed to either 400 ppm gNO or medical-grade air for 7 h. As shown in Fig. 2A, MSH levels decreased steadily to zero by 3.5 h upon exposure to gNO, and MSH levels in the bacterial suspension exposed to medical air slowly increased over 7 h. This result indicates that MSH is being depleted by gNO and further supports a role for MSH in detoxifying the RNIs generated by exposure to gNO. Similar observations have been made for eukaryotic systems where exposure to NO led to respiration inhibition in the mitochondria (2) due to the depletion of mitochondrial GSH (16), and a prolonged exposure to NO was required to overcome these cellular GSH levels.
FIG. 2.
MSH pool in M. smegmatis is decreased by exposure to NO. (A) MSH levels in M. smegmatis mc2155 in 0.9% saline solution decreased with continuous exposure to gNO. Organisms were exposed to 400 ppm gNO (triangles) or medical air (squares) for 7 h. The amount of MSH decreased steadily to almost zero in less than 3.5 h on gNO exposure. (B) MSH levels in M. smegmatis mc2155 decreased with exposure to the NO donor sodium nitrite, while bacterial viability remained relatively unaffected. M. smegmatis mc2155 was grown to log phase in Middlebrook 7H9 media supplemented with 0.05% Tween and 1% glucose (pH 5.5), diluted to 0.5 optical density units at 600 nm in the same medium, and treated with various concentrations of sodium nitrite for 2 hours. Numbers of CFU (bars) and MSH levels (triangles) are shown. (C) MSH levels in M. smegmatis mc2155 decreased with exposure to the NO donor GSNO, while viability remained unaffected. M. smegmatis mc2155 was grown to log phase in Middlebrook 7H9 media supplemented with 0.05% Tween and 1% glucose, diluted to 0.5 optical density units at 600 nm with the same medium, and treated with various amounts of GSNO for 2 h. CFU/ml (bars), GSH levels (circles), and MSH levels (triangles) are shown. Each data point represents the mean ± standard error for four independent measurements.
To determine the NO effect on actively growing M. smegmatis, we also used NO donors in growth media. MSH levels were measured along with viable cell counts after NaNO2 and GSNO (S-nitrosoglutathione) treatments in growth media. Similar to what was found for gNO treatment, MSH levels were depleted before a decrease in bacterial viability was seen (Fig. 2B). A decrease in MSH levels that was dependent on the concentration of GSNO was observed, and this decrease in MSH directly correlated with the amount of GSH resulting from NO release (Fig. 2C).
While nitrosylation of MSH by NO has not yet been demonstrated, Kharitonov et al. described the kinetics of GSNO formation from GSH and NO in the presence of O2 in vitro (7). Under conditions of high NO concentration, S nitrosylation of GSH is favored over protein-bound cysteine residues (6). Our results support a GSH-like role for MSH as the major protectant against NO toxicity in mycobacteria.
Together, these data lead us to suggest the following as the most likely scenario for mycobacterial resistance to continuous gNO exposure. MSH interacts with NO to mitigate this molecule's toxic effects. MSH levels decrease upon exposure to NO, but mycobacteria continue to be viable during early gNO exposure, suggesting that cellular MSH is still available for protection. On prolonged exposure, NO binds to all available MSH and mycobacterial viability decreases sharply as the cellular detoxification system no longer has the capacity to bind NO or NO-generated ROIs and RNIs. This depletion of MSH likely coincides with the end of the latency period. These data, combined with those from our previous studies, provide evidence for MSH as the major detoxification system in mycobacteria for ROIs, RNIs, and antibiotics.
Acknowledgments
This work was funded by Pulmonox Medical Inc. Financial support was provided to Y.A. by the TB Veterans Charitable Foundation and to M.R. by the NIH-MBRS-SCORE 2S06 GM061223-05A1 award. M.R. and Y.A. were also supported by the British Columbia Lung Association.
We thank Barb Conway for assistance with manuscript preparation and Bevin McMullin for technical support.
Footnotes
Published ahead of print on 16 July 2007.
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