Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Apr;175(7):2131–2136. doi: 10.1128/jb.175.7.2131-2136.1993

Evidence for plasmid-encoded virulence factors in the phytopathogenic bacterium Clavibacter michiganensis subsp. michiganensis NCPPB382.

D Meletzus 1, A Bermphol 1, J Dreier 1, R Eichenlaub 1
PMCID: PMC204326  PMID: 8458855

Abstract

The tomato pathogen Clavibacter michiganensis subsp. michiganensis NCPPB382, which causes bacterial wilt, harbors two plasmids pCM1 (27.5 kb) and pCM2 (72 kb). After curing of the plasmids, bacterial derivatives were still proficient in the ability to colonize the host plant and in the production of exopolysaccharides but exhibited a reduced virulence. When one of the two plasmids is lost, there is a significant delay in the development of wilting symptoms after infection and a plasmid-free derivative is not able to induce disease symptoms. By cloning of restriction fragments of both plasmids in the plasmid-free strain CMM100, two DNA fragments which restored the virulent phenotype were identified. Further analysis suggested that a fragment of plasmid pCM1 encodes an endocellulase which is involved in the expression of the pathogenic phenotype.

Full text

PDF
2131

Images in this article

2133Image
on p.2133
2134Image
on p.2134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andro T., Chambost J. P., Kotoujansky A., Cattaneo J., Bertheau Y., Barras F., Van Gijsegem F., Coleno A. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase. J Bacteriol. 1984 Dec;160(3):1199–1203. doi: 10.1128/jb.160.3.1199-1203.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnold W., Pühler A. A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing. Gene. 1988 Oct 15;70(1):171–179. doi: 10.1016/0378-1119(88)90115-1. [DOI] [PubMed] [Google Scholar]
  3. Beck E., Ludwig G., Auerswald E. A., Reiss B., Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene. 1982 Oct;19(3):327–336. doi: 10.1016/0378-1119(82)90023-3. [DOI] [PubMed] [Google Scholar]
  4. Clark M. C., Lawrence C. H. Characterization of a plasmid in isolates of Corynebacterium sepedonicum. Can J Microbiol. 1986 Aug;32(8):617–622. doi: 10.1139/m86-116. [DOI] [PubMed] [Google Scholar]
  5. Ghangas G. S., Hu Y. J., Wilson D. B. Cloning of a Thermomonospora fusca xylanase gene and its expression in Escherichia coli and Streptomyces lividans. J Bacteriol. 1989 Jun;171(6):2963–2969. doi: 10.1128/jb.171.6.2963-2969.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  7. Meletzus D., Eichenlaub R. Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector. J Bacteriol. 1991 Jan;173(1):184–190. doi: 10.1128/jb.173.1.184-190.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mogen B. D., Oleson A. E. Homology of pCS1 Plasmid Sequences with Chromosomal DNA in Clavibacter michiganense subsp. sepedonicum: Evidence for the Presence of a Repeated Sequence and Plasmid Integration. Appl Environ Microbiol. 1987 Oct;53(10):2476–2481. doi: 10.1128/aem.53.10.2476-2481.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roberts D. P., Denny T. P., Schell M. A. Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J Bacteriol. 1988 Apr;170(4):1445–1451. doi: 10.1128/jb.170.4.1445-1451.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schell M. A., Roberts D. P., Denny T. P. Analysis of the Pseudomonas solanacearum polygalacturonase encoded by pglA and its involvement in phytopathogenicity. J Bacteriol. 1988 Oct;170(10):4501–4508. doi: 10.1128/jb.170.10.4501-4508.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Strobel G. A. A phytotoxic glycopeptide from potato plants infected with Corynebacterium sepedonicum. J Biol Chem. 1970 Jan 10;245(1):32–38. [PubMed] [Google Scholar]
  12. Vidaver A. K. The plant pathogenic corynebacteria. Annu Rev Microbiol. 1982;36:495–517. doi: 10.1146/annurev.mi.36.100182.002431. [DOI] [PubMed] [Google Scholar]
  13. Wohlleben W., Arnold W., Bissonnette L., Pelletier A., Tanguay A., Roy P. H., Gamboa G. C., Barry G. F., Aubert E., Davies J. On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette. Mol Gen Genet. 1989 Jun;217(2-3):202–208. doi: 10.1007/BF02464882. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES