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. 1988 Nov;54(11):2599–2602. doi: 10.1128/aem.54.11.2599-2602.1988

Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.

W M Cosby 1, I A Casas 1, W J Dobrogosz 1
PMCID: PMC204341  PMID: 3214148

Abstract

Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfection efficiencies ranged from 25 to 230 PFU/microgram of DNA and from 2.2 X 10(-5) to 4.7 X 10(-4) PFU per recovered protoplast. Total transfectant yield was 3.7 X 10(2) to 3.4 X 10(3) per treatment. Transformations with plasmid DNA were unsuccessful.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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