Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Nov;54(11):2636–2642. doi: 10.1128/aem.54.11.2636-2642.1988

Stability of Bradyrhizobium japonicum Inoculants after Introduction into Soil

Brigitte Brunel 1,*, Jean-Claude Cleyet-Marel 1, Philippe Normand 1, Rene Bardin 1
PMCID: PMC204348  PMID: 16347768

Abstract

Bradyrhizobium japonicum USDA 125-Sp, USDA 138, and USDA 138-Sm had been used as inoculants for soybean (Glycine max (L.) Merr.) in soils previously free of B. japonicum. At 8 to 13 years after their release, these strains were reisolated from soil samples. A total of 115 isolates were obtained through nodules, and seven colonies were obtained directly by a serological method. The stability of the inoculants was confirmed by comparing the reisolated cultures with their respective parental strains which had been preserved by being lyophilized or stored on a yeast extract-mannitol agar slant at 4°C. Comparisons were made on morphological and serological characters, carbon compound utilization (8 tested), intrinsic antibiotic resistance (9 tested), and enzymatic activity (19 tested). Mucous and nonmucous isolates of serogroup 125 were analyzed for symbiotic effectiveness and restriction fragment hybridization with a DNA probe. Our data suggest that the B. japonicum inoculants have survived for up to 13 years in the soils without significant mutation except for two reisolates with a slightly increased kanamycin resistance level.

Full text

PDF
2636

Images in this article

2640Image
on p.2640

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cannon F. C., Riedel G. E., Ausubel F. M. Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized. Mol Gen Genet. 1979 Jul 2;174(1):59–66. doi: 10.1007/BF00433306. [DOI] [PubMed] [Google Scholar]
  2. Herridge D. F., Roughley R. J. Variation in colony characteristics and symbiotic effectiveness of Rhizobium. J Appl Bacteriol. 1975 Feb;38(1):19–27. doi: 10.1111/j.1365-2672.1975.tb00495.x. [DOI] [PubMed] [Google Scholar]
  3. Hranitzky K. W., Larson A. D., Ragsdale D. W., Siebeling R. J. Isolation of O1 serovars of Vibrio cholerae from water by serologically specific method. Science. 1980 Nov 28;210(4473):1025–1026. doi: 10.1126/science.7434013. [DOI] [PubMed] [Google Scholar]
  4. Kuykendall L. D., Elkan G. H. Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. Appl Environ Microbiol. 1976 Oct;32(4):511–519. doi: 10.1128/aem.32.4.511-519.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kuykendall L. D., Elkan G. H. Some features of mannitol metabolism in Rhizobium japonicum. J Gen Microbiol. 1977 Jan;98(1):291–295. doi: 10.1099/00221287-98-1-291. [DOI] [PubMed] [Google Scholar]
  6. Masterson R. V., Prakash R. K., Atherly A. G. Conservation of symbiotic nitrogen fixation gene sequences in Rhizobium japonicum and Bradyrhizobium japonicum. J Bacteriol. 1985 Jul;163(1):21–26. doi: 10.1128/jb.163.1.21-26.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mathis J. N., Barbour W. M., Miller T. B., Israel D. W., Elkan G. H. Characterization of a Mannitol-Utilizing, Nitrogen-Fixing Bradyrhizobium japonicum USDA 110 Derivative. Appl Environ Microbiol. 1986 Jul;52(1):81–85. doi: 10.1128/aem.52.1.81-85.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mathis J. N., Kuykendall L. D., Elkan G. H. Restriction Endonuclease and nif Homology Patterns of Bradyrhizobium japonicum USDA 110 Derivatives With and Without Nitrogen Fixation Competence. Appl Environ Microbiol. 1986 Mar;51(3):477–480. doi: 10.1128/aem.51.3.477-480.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Obaton M. M. Utilisation de mutants spontanés résistants aux antibiotiques pour l'étude écologique des Rhizobium. C R Acad Sci Hebd Seances Acad Sci D. 1971 May 17;272(20):2630–2633. [PubMed] [Google Scholar]
  10. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  11. Sadowsky M. J., Bohlool B. B., Keyser H. H. Serological Relatedness of Rhizobium fredii to Other Rhizobia and to the Bradyrhizobia. Appl Environ Microbiol. 1987 Aug;53(8):1785–1789. doi: 10.1128/aem.53.8.1785-1789.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sadowsky M. J., Tully R. E., Cregan P. B., Keyser H. H. Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean. Appl Environ Microbiol. 1987 Nov;53(11):2624–2630. doi: 10.1128/aem.53.11.2624-2630.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schofield P. R., Gibson A. H., Dudman W. F., Watson J. M. Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population. Appl Environ Microbiol. 1987 Dec;53(12):2942–2947. doi: 10.1128/aem.53.12.2942-2947.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Tautz D., Renz M. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Anal Biochem. 1983 Jul 1;132(1):14–19. doi: 10.1016/0003-2697(83)90419-0. [DOI] [PubMed] [Google Scholar]
  16. Upchurch R. G., Elkan G. H. Comparison of colony morphology, salt tolerance, and effectiveness in Rhizobium japonicum. Can J Microbiol. 1977 Sep;23(9):1118–1122. doi: 10.1139/m77-167. [DOI] [PubMed] [Google Scholar]
  17. van Rensburg H. J., Strijdom B. W. Effectiveness of Rhizobium strains used in inoculants after their introduction into soil. Appl Environ Microbiol. 1985 Jan;49(1):127–131. doi: 10.1128/aem.49.1.127-131.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES