Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1979 Jun;66(2):161–163. doi: 10.1111/j.1476-5381.1979.tb13659.x

Mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes.

M Di Rosa, P Persico
PMCID: PMC2043633  PMID: 465865

Abstract

Hydrocortisone (10 microgram/ml) greatly inhibits the prostaglandin release by rat peritoneal leucocytes phagocytosing killed bacteria. The inhibition, which occurs after an initial latency of 30 min, is completely reversed by either actinomycin D (0.5 microgram/ml) or cycloheximide (1 microgram/ml). Since these antibiotics are known inhibitors of DNA-dependent RNA synthesis and protein synthesis respectively, it appears that the mechanism of inhibition of prostaglandin biosynthesis by hydrocortisone in rat leucocytes involves stimulation of transcription and induction of protein synthesis.

Full text

PDF
161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bray M. A., Gordon D. Effects of anti-inflammatory drugs on macrophage prostaglandin biosynthesis [proceedings]. Br J Pharmacol. 1976 Jul;57(3):466P–467P. [PMC free article] [PubMed] [Google Scholar]
  2. Danon A., Assouline G. Inhibition of prostaglandin biosynthesis by corticosteroids requires RNA and protein synthesis. Nature. 1978 Jun 15;273(5663):552–554. doi: 10.1038/273552a0. [DOI] [PubMed] [Google Scholar]
  3. Floman Y., Zor U. Mechanism of steroid action in inflammation: inhibition of prostaglandin synthesis and release. Prostaglandins. 1976 Sep;12(3):403–413. doi: 10.1016/0090-6980(76)90021-6. [DOI] [PubMed] [Google Scholar]
  4. Flower R. J., Blackwell G. J. The importance of phospholipase-A2 in prostaglandin biosynthesis. Biochem Pharmacol. 1976 Feb 1;25(3):285–291. doi: 10.1016/0006-2952(76)90216-1. [DOI] [PubMed] [Google Scholar]
  5. Gilmore N., Vane J. R., Wyllie J. H. Prostaglandins released by the spleen. Nature. 1968 Jun 22;218(5147):1135–1140. doi: 10.1038/2181135a0. [DOI] [PubMed] [Google Scholar]
  6. Glatt M., Kälin H., Wagner K., Brune K. Prostaglandin release from macrophages: an assay system for anti-inflammatory drugs in vitro. Agents Actions. 1977 Sep;7(3):321–326. doi: 10.1007/BF01969563. [DOI] [PubMed] [Google Scholar]
  7. Gryglewski R. J., Panczenko B., Korbut R., Grodzinska L., Ocetkiewicz A. Corticosteroids inhibit prostaglandin release from perfused mesenteric blood vessels of rabbit and from perfused lungs of sensitized guinea pig. Prostaglandins. 1975 Aug;10(2):343–355. doi: 10.1016/0090-6980(75)90053-2. [DOI] [PubMed] [Google Scholar]
  8. Gryglewski R. J. Steroid hormones, anti-inflammatory steroids and prostaglandins. Pharmacol Res Commun. 1976 Aug;8(4):337–348. doi: 10.1016/0031-6989(76)90034-5. [DOI] [PubMed] [Google Scholar]
  9. Higgs G. A., McCall E., Youlten L. J. A chemotactic role for prostaglandins released from polymorphonuclear leucocytes during phagocytosis. Br J Pharmacol. 1975 Apr;53(4):539–546. doi: 10.1111/j.1476-5381.1975.tb07392.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kantrowitz F., Robinson D. R., McGuire M. B., Levine L. Corticosteroids inhibit prostaglandin production by rheumatiod synovia. Nature. 1975 Dec 25;258(5537):737–739. doi: 10.1038/258737a0. [DOI] [PubMed] [Google Scholar]
  11. Lewis G. P., Piper P. J. Inhibition of release of prostaglandins as an explanation of some of the actions of anti-inflammatory corticosteroids. Nature. 1975 Mar 27;254(5498):308–311. doi: 10.1038/254308a0. [DOI] [PubMed] [Google Scholar]
  12. Parente L., Ammendola G., Persico P., di Rosa M. Glucocorticosteroids, prostaglandins and the inflammatory process. Pol J Pharmacol Pharm. 1978 Mar-Jun;30(2-3):141–155. [PubMed] [Google Scholar]
  13. Spector W. G. The granulomatous inflammatory exudate. Int Rev Exp Pathol. 1969;8:1–55. [PubMed] [Google Scholar]
  14. Tashjian A. H., Jr, Voelkel E. F., McDonough J., Levine L. Hydrocortisone inhibits prostaglandin production by mouse fibrosarcoma cells. Nature. 1975 Dec 25;258(5537):739–741. doi: 10.1038/258739a0. [DOI] [PubMed] [Google Scholar]
  15. Thompson E. B., Lippman M. E. Mechanism of action of glucocorticoids. Metabolism. 1974 Feb;23(2):159–202. doi: 10.1016/0026-0495(74)90113-9. [DOI] [PubMed] [Google Scholar]
  16. Yaron M., Yaron I., Gurari-Rotman D., Revel M., Lindner H. R., Zor U. Stimulation of prostaglandin E production in cultured human fibroblasts by poly(I)-poly(C) and human interferon. Nature. 1977 Jun 2;267(5610):457–459. doi: 10.1038/267457a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES