Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1979 Jun;66(2):211–215. doi: 10.1111/j.1476-5381.1979.tb13667.x

Effect of 5-hydroxytryptamine on protein synthesis in gastrointestinal and other tissues and on serum gastrin concentrations in rats.

A P Nandi Majumdar, A M Nakhla
PMCID: PMC2043643  PMID: 465872

Abstract

1 The effect of 5-hydroxytryptamine (5-HT) on protein synthesis in the gastrointestinal tissues as well as in the liver, heart and brain was studied by measuring [3H]-leucine incorporation into total tissue protein in vivo in rats. 2 A single injection of 5-HT (10 mg/kg) produced a marked inhibition (45 to 65%) in protein synthesis in the stomach (oxyntic gland area), intestine (jejunum + ileum), colon and brain, but not in the liver and heart. 3 Dose- and time-dependent studies of 5-HT-induced changes in protein synthesis in the stomach of fed rats revealed that the maximal inhibition of about 65% occurred 1 h after a dose of 12.5 mg/kg. 4 Animals deprived of food for 24 h differed from their fed counterparts only in the degree of responsiveness, in that a greater inhibition (75%) of [3H]-leucine incorporation into total protein of the stomach was observed 30 min after 5-HT injection. 5 Pretreatment of animals with methysergide (0.25 mg/kg) essentially abolished the 5-HT-mediated inhibition of protein synthesis in the stomach. 6 Serum gastrin concentration in fasted animals remained slightly elevated during the initial period of 5-HT treatment (up to 1 h). 7 The results demonstrate that in certain tissues, 5-HT markedly inhibits protein synthesis. The inhibition in protein synthesis in the gastrointestinal tract cannot be attributed to changes in the concentration of gastrin.

Full text

PDF
211

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DOLCINI H. A., ZAIDMAN I., GRAY S. J. Hormonal and pharmacologic influences on microcirculation in the rat stomach. Am J Physiol. 1960 Dec;199:1157–1160. doi: 10.1152/ajplegacy.1960.199.6.1157. [DOI] [PubMed] [Google Scholar]
  2. Hano J., Bugajski J., Danek L., Wantuch C. The influence of serotonin on insulin-stimulated gastric secretion, blood glucose and serum electrolyte levels in the unanesthetized rat. Arch Int Pharmacodyn Ther. 1975 Jul;216(1):28–39. [PubMed] [Google Scholar]
  3. Håkanson R., Lilja B., Owman C., Thunell S. Changes in gastric secretion induced by certain amines, amine precursors and related enzyme inhibitors. Eur J Pharmacol. 1967 Sep;1(5):425–433. doi: 10.1016/0014-2999(67)90105-7. [DOI] [PubMed] [Google Scholar]
  4. Jaffe B. M., Kopen D. F., Lazan D. W. Endogenous serotonin in the control of gastric acid secretion. Surgery. 1977 Jul;82(1):156–163. [PubMed] [Google Scholar]
  5. Johnson L. R., Aures D., Yuen L. Pentagastrin-induced stimulation of protein synthesis in the gastrointestinal tract. Am J Physiol. 1969 Jul;217(1):251–254. doi: 10.1152/ajplegacy.1969.217.1.251. [DOI] [PubMed] [Google Scholar]
  6. Johnson L. R. The trophic action of gastrointestinal hormones. Gastroenterology. 1976 Feb;70(2):278–288. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Majumdar A. P., Goltermann N. Chronic administration of pentagastrin: effect on gastric mucosal protein synthesis in rats. Mol Cell Endocrinol. 1978 Jul-Aug;11(2):137–143. doi: 10.1016/0303-7207(78)90002-3. [DOI] [PubMed] [Google Scholar]
  9. Majumdar A. P., Goltermann N. Effects of fasting and pentagastrin on protein synthesis by isolated gastric mucosal ribosomes in a cell-free system. Gastroenterology. 1977 Nov;73(5):1060–1064. [PubMed] [Google Scholar]
  10. Nandi Majumdar A. P., Fjendbo Jorgensen A. J. Response of well-fed adrenalectomized rats to tryptophan force-feeding on hepatic protein and RNA synthesis. Biochem Med. 1976 Dec;16(3):266–276. doi: 10.1016/0006-2944(76)90032-6. [DOI] [PubMed] [Google Scholar]
  11. Nandi Majumdar A. P., Greenfield S., Röigaard-Petersen H. Metabolism of plasma albumin in rats with congenital hyperbilirubinemia (Gunn). Scand J Clin Lab Invest. 1973 Mar;31(2):219–224. doi: 10.3109/00365517309084313. [DOI] [PubMed] [Google Scholar]
  12. RESNICK R. H., GRAY S. J. Chemical and histologic demonstration of hydrochloric acid-induced release of serotonin from intestinal mucosa. Gastroenterology. 1962 Jan;42:48–55. [PubMed] [Google Scholar]
  13. Rehfeld J. F., Stadil F., Rubin B. Production and evaluation of antibodies for the radioimmunoassay of gastrin. Scand J Clin Lab Invest. 1972 Oct;30(2):221–232. doi: 10.3109/00365517209081114. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES