Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1979 Jun;66(2):223–228. doi: 10.1111/j.1476-5381.1979.tb13669.x

Relationship between accumulation, storage and overflow of noradrenaline in the rat salivary gland after chronic treatment with guanethidine.

M T Khan, A R Wakade
PMCID: PMC2043645  PMID: 465874

Abstract

1 The effect of guanethidine on the endogenous noradrenaline (NA) content, accumulation and overflow of [3H]-noradrenaline ([3H]-NA) in the rat salivary gland was examined at various times after drug administration. 2 Twenty-four h after a single injection of guanethidine (1 or 10 mg/kg s.c.), the respective values for the endogenous NA content and for the accumulation and overflow of [3H]-NA were approximately 55, 85, 30%, and 15, 55, 10% of the controls. 3 Although [3H]-NA accumulation had returned to control levels within 48 h after the dose of 10 mg/kg guanethidine, the overflow of [3H]-NA evoked by electrical stimulation or excess potassium (K+) remained depressed. 4 After the low or the high dose of guanethidine, the NA content of the salivary gland was restored to about 50% of the normal value between 4 to 24 and 48 to 72 h, respectively. 5 The accumulation of [3H]-NA was inhibited by about 75% by cocaine. The same degree of inhibition was obtained 4 h after 10 mg/kg guanethidine. In these experiments phenoxybenzamine did not reduce the residual (25%) uptake. 6 The reasons for differential rates of recovery of the endogenous NA content and the storage of [3H]-NA after guanethidine are discussed.

Full text

PDF
223

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avakian O. V., Gillespie J. S. Uptake of noradrenaline by adrenergic nerves, smooth muscle and connective tissue in isolated perfused arteries and its correlation with the vasoconstrictor response. Br J Pharmacol Chemother. 1968 Jan;32(1):168–184. doi: 10.1111/j.1476-5381.1968.tb00441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASS R., SPRIGGS T. L. Tissue amine levels and sympathetic blockade after guanethidine and bretylium. Br J Pharmacol Chemother. 1961 Dec;17:442–450. doi: 10.1111/j.1476-5381.1961.tb01131.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANG C. C., COSTA E., BRODIE B. B. INTERACTION OF GUANETHIDINE WITH ADRENERGIC NEURONS. J Pharmacol Exp Ther. 1965 Mar;147:303–312. [PubMed] [Google Scholar]
  4. Dixon W. R., Garcia A. G., Kirpekar S. M. Release of catecholamines and dopamine beta-hydroxylase from the perfused adrenal gland of the cat. J Physiol. 1975 Jan;244(3):805–824. doi: 10.1113/jphysiol.1975.sp010827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Durant G. J., Roe A. M., Green A. L. The chemistry of guanidines and their actions at adrenergic nerve endings. Prog Med Chem. 1970;7(1):124–213. doi: 10.1016/s0079-6468(08)70353-9. [DOI] [PubMed] [Google Scholar]
  6. Fielden R., Green A. L. A comparative study of the noradrenaline-depleting and sympathetic-blocking actions of guanethidine and (-)-beta-hydroxyphenethylguanidine. Br J Pharmacol Chemother. 1967 May;30(1):155–165. doi: 10.1111/j.1476-5381.1967.tb02121.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furchgott R. F., Sanchez Garcia P. Effects of inhibition of monoamine oxidase on the actions and interactions of norepinephrine, tyramine and other drugs on guinea-pig left atrium. J Pharmacol Exp Ther. 1968 Sep;163(1):98–122. [PubMed] [Google Scholar]
  8. GAFFNEY T. E., CHIDSEY C. A., BRAUNWALD E. Study of the relationship between the neurotransmitter store and adrenergic nerve block induced by reserpine and guanethidine. Circ Res. 1963 Mar;12:264–268. doi: 10.1161/01.res.12.3.264. [DOI] [PubMed] [Google Scholar]
  9. HERTING G., AXELROD J., WHITBY L. G. Effect of drugs on the uptake and metabolism of H3-norepinephrine. J Pharmacol Exp Ther. 1961 Nov;134:146–153. [PubMed] [Google Scholar]
  10. HERTTING G., POTTER L. T., AXELROD J. Effect of decentralization and ganglionic blocking agents on the spontaneous release of H3-norepinephrine. J Pharmacol Exp Ther. 1962 Jun;136:289–292. [PubMed] [Google Scholar]
  11. Kirpekar S. M., Furchgott R. F. Interaction of tyramine and guanethidine in the spleen of the cat. J Pharmacol Exp Ther. 1972 Jan;180(1):38–46. [PubMed] [Google Scholar]
  12. Kirpekar S. M., Wakade A. R., Dixon W., Prat J. C. Effect of cocaine, phenoxybenzamine and calcium on the inhibition of norepinephrine output from the cat spleen by guanthidine. J Pharmacol Exp Ther. 1969 Feb;165(2):166–175. [PubMed] [Google Scholar]
  13. Lundborg P., Stitzel R. E. Studies on the dual action of guanethidine in sympathetic nerves. Acta Physiol Scand. 1968 Jan-Feb;72(1):100–107. doi: 10.1111/j.1748-1716.1968.tb03831.x. [DOI] [PubMed] [Google Scholar]
  14. Shellenberger M. K., Gordon J. H. A rapid, simplified procedure for simultaneous assay of norepinephrine, dopamine, and 5-hydroxytryptamine from discrete brain areas. Anal Biochem. 1971 Feb;39(2):356–372. doi: 10.1016/0003-2697(71)90426-x. [DOI] [PubMed] [Google Scholar]
  15. Spriggs T. L. Peripheral noradrenaline and adrenergic transmission in the rat. Br J Pharmacol Chemother. 1966 Jan;26(1):271–281. doi: 10.1111/j.1476-5381.1966.tb01830.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wakade A. R., Furchgott R. F. Metabolic requirements for the uptake and storage of norepinephrine by the isolated left atrium of the guinea pig. J Pharmacol Exp Ther. 1968 Sep;163(1):123–135. [PubMed] [Google Scholar]
  17. Wakade A. R., Kirpekar S. M. Calcium-independent release of 3H-norepinephrine from reserpine-pretreated guinea-pig vas deferens and seminal vesicle. J Pharmacol Exp Ther. 1974 Sep;190(3):451–458. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES