Abstract
A bacterial strain that produces d-aminoacylase was isolated from soil and identified as Alcaligenes denitrificans subsp. xylosoxydans MI-4. l-Aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-Aminoacylase was inducibly produced. N-Acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. Enzymatic resolution of N-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was approximately 100/7, suggesting that resolution with crude enzymes may become possible by removing small amounts of the contaminated l-form with l-amino acid oxidase.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KAMEDA Y., TOYOURA E., YAMAZOE H., KIMURA Y., YASUDA Y. Hydrolysis and metabolism by soil bacteria of benzoyl derivatives of D- and L-forms of some amino-acids. Nature. 1952 Nov 22;170(4334):888–889. doi: 10.1038/170888a0. [DOI] [PubMed] [Google Scholar]
- Kameda Y., Hase T., Kanatomo S., Kita Y. Studies on acylase activity and micro-organisms. XXVI. Purification from AAA 6029 (Pseudomonase sp). Chem Pharm Bull (Tokyo) 1978 Sep;26(9):2698–2704. doi: 10.1248/cpb.26.2698. [DOI] [PubMed] [Google Scholar]
- Kubo K., Ishikura T., Fukagawa Y. Deacetylation of PS-5, a new beta-lactam compound. II. Separation and purification of L- and D-amino acid acylases from Pseudomonas sp. 1158. J Antibiot (Tokyo) 1980 Jun;33(6):550–555. doi: 10.7164/antibiotics.33.550. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Moriguchi M., Hara Y., Hatanaka S. Antibacterial activity of L-2-amino-4-chloro-4-pentenoic acid isolated from Amanita pseudoporphyria Hongo. J Antibiot (Tokyo) 1987 Jun;40(6):904–906. doi: 10.7164/antibiotics.40.904. [DOI] [PubMed] [Google Scholar]
- Tsai Y. C., Tseng C. P., Hsiao K. M., Chen L. Y. Production and Purification of d-Aminoacylase from Alcaligenes denitrificans and Taxonomic Study of the Strain. Appl Environ Microbiol. 1988 Apr;54(4):984–989. doi: 10.1128/aem.54.4.984-989.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]