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. 1988 Nov;54(11):2838–2841. doi: 10.1128/aem.54.11.2838-2841.1988

Comparison of rapid NFT and API 20E with conventional methods for identification of gram-negative nonfermentative bacilli from pharmaceuticals and cosmetics.

M J Palmieri 1, S L Carito 1, R F Meyer 1
PMCID: PMC204382  PMID: 3214161

Abstract

The accuracy of the Rapid NFT and the API 20E identification systems was evaluated by comparing them with conventional biochemical methods for the identification of gram-negative, nonfermentative bacilli. The organisms were recovered from preserved, nonsterile pharmaceutical and cosmetic products. A total of 123 test isolates that are commonly encountered in these products were used. By using the criteria of accurate and reliable identification without employing additional tests, Rapid NFT was found to be more accurate after 48 h of incubation than API 20E for characterizing isolates to the species level. Therefore, close agreement between NFT and conventional methods for identification of industrial gram-negative isolates provides evidence that the Rapid NFT system is an improved and rapid method for identifying these organisms to the species level with minimal use of supplementary tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Leathers D. J. Evaluation of the rapid NFT system for identification of gram-negative, nonfermenting rods. J Clin Microbiol. 1984 Oct;20(4):730–734. doi: 10.1128/jcm.20.4.730-734.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnishan J., Ayers L. W. Rapid identification of nonfermentative gram-negative rods by the Corning N/F system. J Clin Microbiol. 1979 Feb;9(2):239–243. doi: 10.1128/jcm.9.2.239-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruckner D. A., Clark V., Martin W. J. Comparison of Enteric-Tek with API 20E and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1982 Jan;15(1):16–18. doi: 10.1128/jcm.15.1.16-18.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark W. A. A simplified Leifson flagella stain. J Clin Microbiol. 1976 Jun;3(6):632–634. doi: 10.1128/jcm.3.6.632-634.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Isenberg H. D., Sampson-Scherer J. Clinical laboratory evaluation of a system approach to the recognition of nonfermentative or oxidase-producing gram-negative, rod-shaped bacteria. J Clin Microbiol. 1977 Mar;5(3):336–340. doi: 10.1128/jcm.5.3.336-340.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Martin R., Siavoshi F., McDougal D. L. Comparison of Rapid NFT system and conventional methods for identification of nonsaccharolytic gram-negative bacteria. J Clin Microbiol. 1986 Dec;24(6):1089–1092. doi: 10.1128/jcm.24.6.1089-1092.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oberhofer T. R., Rowen J. W., Cunningham G. F. Characterization and identification of gram-negative, nonfermentative bacteria. J Clin Microbiol. 1977 Feb;5(2):208–220. doi: 10.1128/jcm.5.2.208-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Otto L. A., Pickett M. J. Rapid method for identification of gram-negative, nonfermentative bacilli. J Clin Microbiol. 1976 Jun;3(6):566–575. doi: 10.1128/jcm.3.6.566-575.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shayegani M., Maupin P. S., McGlynn D. M. Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria. J Clin Microbiol. 1978 Jun;7(6):539–545. doi: 10.1128/jcm.7.6.539-545.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Søgaard P., Gahrn-Hansen B., Zhou H. P., Frederiksen W. An investigation of three commercial methods for rapid identification of non-enteric gram-negative rods. Application on Pseudomonas paucimobilis and some other Pseudomonas species. Acta Pathol Microbiol Immunol Scand B. 1986 Oct;94(5):357–363. doi: 10.1111/j.1699-0463.1986.tb03067.x. [DOI] [PubMed] [Google Scholar]

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