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. 1979 Oct;67(2):247–254. doi: 10.1111/j.1476-5381.1979.tb08673.x

Presynaptic inhibitory action of 5-hydroxytryptamine in dog isolated saphenous vein

W Feniuk, PPA Humphrey, AD Watts
PMCID: PMC2043877  PMID: 497528

Abstract

1 The effect of 5-hydroxytryptamine on contractile responses to sympathetic nerve stimulation has been studied in the dog isolated saphenous vein.

2 Electrical stimulation (0.1 to 10 Hz) of dog saphenous vein strips produced frequency-dependent contractions. Contractions produced by stimulation at 2 Hz were almost completely blocked by tetrodotoxin (3.1 × 10-8 mol/l) or phentolamine (5.0 × 10-6 mol/l) but mecamylamine (5.0 × 10-6 mol/l) had little effect. This suggests that the contractions were mediated predominantly through noradrenaline release from postganglionic noradrenergic nerves.

3 Contractions produced by intermittent electrical stimulation at 2 Hz were inhibited by 5-hydroxy-tryptamine (1.0 × 10-9 to 1.0 × 10-7 mol/l) in a concentration-dependent manner whilst contractions induced by exogenous noradrenaline were not affected.

4 The inhibitory action of 5-hydroxytryptamine was most marked at low frequencies of stimulation and with low pulse numbers.

5 High external calcium concentrations (3.9 and 5.2 × 10-3 mol/l) reduced the inhibitory action of 5-hydroxytryptamine.

6 Cyproheptadine (1.0 × 10-8 mol/l to 1.0 × 10-6 mol/l) or morphine (1.0 × 10-7 mol/l to 1.0 × 10-5 mol/l) did not antagonize the inhibitory action of 5-hydroxytryptamine. Methysergide (1.0 × 10-7 mol/l) slightly reduced the contractions produced by electrical stimulation and only weakly antagonized the action of 5-hydroxytryptamine.

7 It is suggested that a 5-hydroxytryptamine receptor exists presynaptically in the dog isolated saphenous vein strip and that stimulation of this receptor by low concentrations of 5-hydroxytryptamine inhibits the release of noradrenaline from noradrenergic nerves. This receptor type is resistant to blockade by `classical' 5-hydroxytryptamine antagonists.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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