Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Nov;54(11):2866–2867. doi: 10.1128/aem.54.11.2866-2867.1988

Improved Assay for Rhodanese in Thiobacillus spp

David R Singleton 1,†, David W Smith 1,*
PMCID: PMC204388  PMID: 16347783

Abstract

Rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Conventional rhodanese assays colorimetrically measure the formation of one or the other of the products. These assays suffer from the fact that there is significant nonbiological formation of these products in addition to the enzymatically catalyzed reaction. In the present report, we describe a modified procedure for assaying rhodanese in which a separate boiled control was prepared for each assay trial. The boiled control corrected for the nonbiological contributions to product formation.

Full text

PDF
2866

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agrò A. F., Cannella C., Graziani M. T., Cavallini D. A possible role for rhodanese: The formation of 'labile' sulfur from thiosulfate. FEBS Lett. 1971 Aug 15;16(3):172–174. doi: 10.1016/0014-5793(71)80124-2. [DOI] [PubMed] [Google Scholar]
  2. Bonomi F., Pagani S., Cerletti P. Insertion of sulfide into ferredoxins catalyzed by rhodanese. FEBS Lett. 1977 Dec 1;84(1):149–152. doi: 10.1016/0014-5793(77)81076-4. [DOI] [PubMed] [Google Scholar]
  3. Bowen T. J., Butler P. J., Happold F. C. Some properties of the rhodanese system of Thiobacillus denitrificans. Biochem J. 1965 Dec;97(3):651–657. doi: 10.1042/bj0970651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fan D. P., Gardner-Eckstrom G. L. Passage of a membrane protein through the walls of toluene-treated Bacillus megaterium cells. J Bacteriol. 1975 Aug;123(2):717–723. doi: 10.1128/jb.123.2.717-723.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LONDON J., RITTENBERG S. C. PATH OF SULFUR IN SULFIDE AND THIOSULFATE OXIDATION BY THIOBACILLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1183–1190. doi: 10.1073/pnas.52.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pagani S., Eldridge M., Eady R. R. Nitrogenase of Klebsiella pneumoniae. Rhodanese-catalysed restoration of activity of the inactive 2Fe species of the Fe protein. Biochem J. 1987 Jun 1;244(2):485–488. doi: 10.1042/bj2440485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ryan R. W., Gourlie M. P., Tilton R. C. Release of rhodanese from Pseudomonas aeruginosa by cold shock and its localization within the cell. Can J Microbiol. 1979 Mar;25(3):340–351. doi: 10.1139/m79-054. [DOI] [PubMed] [Google Scholar]
  8. Silver S., Wendt L. Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier. J Bacteriol. 1967 Feb;93(2):560–566. doi: 10.1128/jb.93.2.560-566.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smith A. J., Lascelles J. Thiosulphate metabolism and rhodanese in Chromatium sp. strain D. J Gen Microbiol. 1966 Mar;42(3):357–370. doi: 10.1099/00221287-42-3-357. [DOI] [PubMed] [Google Scholar]
  10. Suzuki I. Mechanisms of inorganic oxidation and energy coupling. Annu Rev Microbiol. 1974;28(0):85–101. doi: 10.1146/annurev.mi.28.100174.000505. [DOI] [PubMed] [Google Scholar]
  11. VILLAREJO M., WESTLEY J. MECHANISM OF RHODANESE CATALYSIS OF THIOSULFATE-LIPOATE OXIDATION-REDUCTION. J Biol Chem. 1963 Dec;238:4016–4020. [PubMed] [Google Scholar]
  12. Westley J. Rhodanese. Adv Enzymol Relat Areas Mol Biol. 1973;39:327–368. doi: 10.1002/9780470122846.ch5. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES