Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1980 May;69(1):107–112. doi: 10.1111/j.1476-5381.1980.tb10888.x

Adenosine inhibition of gamma-aminobutyric acid release from slices of rat cerebral cortex.

C Hollins, T W Stone
PMCID: PMC2044167  PMID: 7378648

Abstract

1 The effect of purine compounds on the potassium-evoked release of 14C-labelled gamma-aminobutyric acid (GABA) has been studied in 400 micrometers slices of rat cerebral cortex in vitro. 2 Adenosine and adenosine 5' monophosphate (AMP) inhibited the release of GABA at 10(-5) to 10(-3) M. Adenosine triphosphate (ATP) produced a significant inhibition of release only at 10(-3) M. 3 Theophylline 10(-4) or 10(-3) M reduced the inhibitory effect of adenosine, but did not change basal release of GABA. 4 Dipyridamole 10(-5) M itself reduced evoked GABA release, but did not prevent the inhibitory effect of adenosine, implying that adenosine was acting at an extracellularly directed receptor. 5 Calcium removal or antagonism by verapamil reduced the evoked release of GABA, but adenosine did not produce any further reduction of the calcium-independent release. This may indicate that the inhibitory effect of adenosine on GABA release results from interference with calcium influx or availability within the terminals.

Full text

PDF
107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blume A. J., Dalton C., Sheppard H. Adenosine-mediated elevation of cyclic 3':5'-adenosine monophosphate concentrations in cultured mouse neuroblastoma cells. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3099–3102. doi: 10.1073/pnas.70.11.3099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowery N. G., Brown D. A. Proceedings: On the release of accumulated (3-H)-gamma-aminobutyric acid (GABA) from isolated rat superior cervical ganglia. Br J Pharmacol. 1974 Nov;52(3):436P–437P. [PMC free article] [PubMed] [Google Scholar]
  3. Burnstock G. Purinergic nerves. Pharmacol Rev. 1972 Sep;24(3):509–581. [PubMed] [Google Scholar]
  4. Clanachan A. S., Johns A., Paton D. M. Presynaptic inhibitory actions of adenine nucleotides and adenosine on neurotransmission in the rat vas deferens. Neuroscience. 1977;2(4):597–602. doi: 10.1016/0306-4522(77)90056-2. [DOI] [PubMed] [Google Scholar]
  5. Dreifuss J. J., Kelly J. S., Krnjević K. Cortical inhibition and gamma-aminobutyric acid. Exp Brain Res. 1969;9(2):137–154. doi: 10.1007/BF00238327. [DOI] [PubMed] [Google Scholar]
  6. Enero M. A., Saidman B. Q. Possible feed-back inhibition of noradrenaline release by purine compounds. Naunyn Schmiedebergs Arch Pharmacol. 1977 Mar;297(1):39–46. doi: 10.1007/BF00508808. [DOI] [PubMed] [Google Scholar]
  7. Ginsborg B. L., Hirst G. D. The effect of adenosine on the release of the transmitter from the phrenic nerve of the rat. J Physiol. 1972 Aug;224(3):629–645. doi: 10.1113/jphysiol.1972.sp009916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gustafsson L., Hedqvist P., Fredholm B. B., Lundgren G. Inhibition of acetylcholine release in guinea pig ileum by adenosine. Acta Physiol Scand. 1978 Dec;104(4):469–478. doi: 10.1111/j.1748-1716.1978.tb06302.x. [DOI] [PubMed] [Google Scholar]
  9. Harms H. H., Wardeh G., Mulder A. H. Adenosine modulates depolarization-induced release of 3H-noradrenaline from slices of rat brain neocortex. Eur J Pharmacol. 1978 Jun 1;49(3):305–308. doi: 10.1016/0014-2999(78)90107-3. [DOI] [PubMed] [Google Scholar]
  10. Haycock J. W., Levy W. B., Denner L. A., Cotman C. W. Effects of elevated [K+]O on the release of neurotransmitters from cortical synaptosomes: efflux or secretion? J Neurochem. 1978 May;30(5):1113–1125. doi: 10.1111/j.1471-4159.1978.tb12406.x. [DOI] [PubMed] [Google Scholar]
  11. Hedqvist P., Fredholm B. B. Effects of adenosine on adrenergic neurotransmission; prejunctional inhibition and postjunctional enhancement. Naunyn Schmiedebergs Arch Pharmacol. 1976 Jun;293(3):217–223. doi: 10.1007/BF00507344. [DOI] [PubMed] [Google Scholar]
  12. Kuroda Y., Saito M., Kobayashi K. Concomitant changes in cyclic AMP level and postsynaptic potentials of olfactory cortex slices induced by adenosine derivatives. Brain Res. 1976 Jun 4;109(1):196–201. doi: 10.1016/0006-8993(76)90393-0. [DOI] [PubMed] [Google Scholar]
  13. Lekić D. Presynaptic depression of synaptic response of Renshaw cells by adenosine 5'-monophosphate. Can J Physiol Pharmacol. 1977 Dec;55(6):1391–1393. doi: 10.1139/y77-186. [DOI] [PubMed] [Google Scholar]
  14. McIlwain H. Extended roles in the brain for second-messenger systems. Neuroscience. 1977;2(3):357–372. doi: 10.1016/0306-4522(77)90002-1. [DOI] [PubMed] [Google Scholar]
  15. McIlwain H. Regulatory significance of the release and action of adenine derivatives in cerebral systems. Biochem Soc Symp. 1972;(36):69–85. [PubMed] [Google Scholar]
  16. Moritoki H., Kanbe T., Maruoka M., Ohara M., Ishida Y. Potentiation by dipyridamole of the inhibition of guinea-pig ileum twitch response caused by adenine derivatives. J Pharmacol Exp Ther. 1978 Feb;204(2):343–350. [PubMed] [Google Scholar]
  17. Olsson R. A., Davis C. J., Khouri E. M., Patterson R. E. Evidence for an adenosine receptor on the surface of dog coronary myocytes. Circ Res. 1976 Jul;39(1):93–98. doi: 10.1161/01.res.39.1.93. [DOI] [PubMed] [Google Scholar]
  18. Phillis J. W., Kostopoulos G. K. Adenosine as a putative transmitter in the cerebral cortex. Studies with potentiators and antagonists. Life Sci. 1975 Oct 10;17(7):1085–1094. doi: 10.1016/0024-3205(75)90329-x. [DOI] [PubMed] [Google Scholar]
  19. Ribeiro J. A., Walker J. The effects of adenosine triphosphate and adenosine diphosphate on transmission at the rat and frog neuromuscular junctions. Br J Pharmacol. 1975 Jun;54(2):213–218. doi: 10.1111/j.1476-5381.1975.tb06931.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sattin A., Rall T. W. The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices. Mol Pharmacol. 1970 Jan;6(1):13–23. [PubMed] [Google Scholar]
  21. Sattin A., Rall T. W., Zanella J. Regulation of cyclic adenosine 3',5'-monophosphate levels in guinea-pig cerebral cortex by interaction of alpha adrenergic and adenosine receptor activity. J Pharmacol Exp Ther. 1975 Jan;192(1):22–32. [PubMed] [Google Scholar]
  22. Sawynok J., Jhamandas K. H. Inhibition of acetylcholine release from cholinergic nerves by adenosine, adenine nucleotides and morphine: antagonism by theophylline. J Pharmacol Exp Ther. 1976 May;197(2):379–390. [PubMed] [Google Scholar]
  23. Schrader J., Nees S., Gerlach E. Evidence for a cell surface adenosine receptor on coronary myocytes and atrial muscle cells. Studies with an adenosine derivative of high molecular weight. Pflugers Arch. 1977 Jul 19;369(3):251–257. doi: 10.1007/BF00582192. [DOI] [PubMed] [Google Scholar]
  24. Schrader J., Rubio R., Berne R. M. Inhibition of slow action potentials of guinea pig atrial muscle by adenosine: a possible effect on Ca2+ influx. J Mol Cell Cardiol. 1975 Jun;7(6):427–433. doi: 10.1016/0022-2828(75)90048-6. [DOI] [PubMed] [Google Scholar]
  25. Srinivasan V., Neal M. J., Mitchell J. F. The effect of electrical stimulation and high potassium concentrations on the efflux of (3H) gamma-aminobutyric acid from brain slices. J Neurochem. 1969 Aug;16(8):1235–1244. doi: 10.1111/j.1471-4159.1969.tb05971.x. [DOI] [PubMed] [Google Scholar]
  26. Stone T. W. Possible roles for purine compounds in neuronal adaptation. Biochem Soc Trans. 1978;6(5):858–862. doi: 10.1042/bst0060858. [DOI] [PubMed] [Google Scholar]
  27. Stone T. W., Taylor D. A. Microiontophoretic studies of the effects of cylic nucleotides on excitability of neurones in the rat cerebral cortex. J Physiol. 1977 Apr;266(3):523–543. doi: 10.1113/jphysiol.1977.sp011780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Su C. Purinergic inhibition of adrenergic transmission in rabbit blood vessels. J Pharmacol Exp Ther. 1978 Feb;204(2):351–361. [PubMed] [Google Scholar]
  29. Subbarao K., Rucinski B., Rausch M. A., Schmid K., Niewiarowski S. Binding of dipyridamole to human platelets and to alpha1 acid glycoprotein and its significance for the inhibition of adenosine uptake. J Clin Invest. 1977 Oct;60(4):936–943. doi: 10.1172/JCI108848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Taylor D. A., Stone T. W. Neuronal responses to extracellularly applied cyclic AMP:Role of the adenosine receptor. Experientia. 1978 Apr 15;34(4):481–482. doi: 10.1007/BF01935940. [DOI] [PubMed] [Google Scholar]
  31. Verhaeghe R. H., Vanhoutte P. M., Shepherd J. T. Inhibition of sympathetic neurotransmission in canine blood vessels by adenosine and adenine nucleotides. Circ Res. 1977 Feb;40(2):208–215. doi: 10.1161/01.res.40.2.208. [DOI] [PubMed] [Google Scholar]
  32. Vizi E. S., Knoll J. The inhibitory effect of adenosine and related nucleotides on the release of acetylcholine. Neuroscience. 1976;1(5):391–398. doi: 10.1016/0306-4522(76)90132-9. [DOI] [PubMed] [Google Scholar]
  33. Wahl M., Kuschinsky W. The dilatatory action of adenosine on pial arteries of cats and its inhibition by theophylline. Pflugers Arch. 1976 Mar 11;362(1):55–59. doi: 10.1007/BF00588681. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES