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. 1988 Dec;54(12):3156–3161. doi: 10.1128/aem.54.12.3156-3161.1988

Production and Further Characterization of an Alkaline Elastase Produced by Alkalophilic Bacillus Strain Ya-B

Ying-Chieh Tsai 1,*, Ray-Yeng Juang 1, Sheun-Fuh Lin 1, Shiow-Wen Chen 1, Makari Yamasaki 1, Gakuzo Tamura 1
PMCID: PMC204442  PMID: 16347804

Abstract

The characteristics of the obligate alkalophilic Bacillus sp. strain Ya-B, which produces alkaline elastase extracellularly, were examined. This strain grew at pH 7.0 only in the presence of 1% or more NaCl. Its fatty acid distribution pattern was similar to that of other Bacillus species in which iso-C15 and anteiso-C15 were the most abundant fatty acids. About 120 mg of enzyme was recovered from 1 liter of culture broth in a medium (pH 10.1) containing mainly glucose, soymeal, and glycerol. The antiserum against this enzyme did not recognize microbial proteinases, such as subtilisins, but reacted with proteinase C, which was purified from commercial pronase. Chemical modification studies revealed that certain histidine and tyrosine residues might be involved in the enzyme activity. This enzyme underwent a partial unfolding at pHs higher than 12.0, as indicated by the circular dichroism study.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aono R., Horikoshi K., Goto S. Composition of the peptidoglycan of alkalophilic Bacillus spp. J Bacteriol. 1984 Feb;157(2):688–689. doi: 10.1128/jb.157.2.688-689.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clejan S., Krulwich T. A., Mondrus K. R., Seto-Young D. Membrane lipid composition of obligately and facultatively alkalophilic strains of Bacillus spp. J Bacteriol. 1986 Oct;168(1):334–340. doi: 10.1128/jb.168.1.334-340.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Guffanti A. A., Finkelthal O., Hicks D. B., Falk L., Sidhu A., Garro A., Krulwich T. A. Isolation and characterization of new facultatively alkalophilic strains of Bacillus species. J Bacteriol. 1986 Sep;167(3):766–773. doi: 10.1128/jb.167.3.766-773.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johansen J. T., Ottesen M., Svendsen I. Chemical derivatives of subtilisin Carlsberg with increased proteolytic activity. Biochim Biophys Acta. 1967 May 16;139(1):211–214. doi: 10.1016/0005-2744(67)90138-6. [DOI] [PubMed] [Google Scholar]
  5. Kaneda T. Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species. J Bacteriol. 1967 Mar;93(3):894–903. doi: 10.1128/jb.93.3.894-903.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Liu T. Y., Chang Y. H. Hydrolysis of proteins with p-toluenesulfonic acid. Determination of tryptophan. J Biol Chem. 1971 May 10;246(9):2842–2848. [PubMed] [Google Scholar]
  7. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  8. Narahashi Y., Shibuya K., Yanagita M. Studies on proteolytic enzymes (pronase) of Streptomyces griseus K-1. II. Separation of exo- and endopeptidases of pronase. J Biochem. 1968 Oct;64(4):427–437. doi: 10.1093/oxfordjournals.jbchem.a128914. [DOI] [PubMed] [Google Scholar]
  9. Otsuki K., Liu C. L., Hatano H. Inactivation of subtilism BPN' by N-bromosuccinimide. J Biochem. 1969 Dec;66(6):863–865. doi: 10.1093/oxfordjournals.jbchem.a129217. [DOI] [PubMed] [Google Scholar]
  10. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  11. Tsai Y. C., Lin Y. T., Yang Y. B., Li Y. F., Yamasaki M., Tamura G. Specificity of alkaline elastase Bacillus on the oxidized insulin A- and B-chains. J Biochem. 1988 Sep;104(3):416–420. doi: 10.1093/oxfordjournals.jbchem.a122482. [DOI] [PubMed] [Google Scholar]
  12. Tsai Y. C., Yamasaki M., Tamura G. Substrate specificity of a new alkaline elastase from an alkalophilic bacillus. Biochem Int. 1984 Feb;8(2):283–288. [PubMed] [Google Scholar]
  13. Tsai Y. C., Yamasaki M., Yamamoto-Suzuki Y., Tamura G. A new alkaline elastase of an alkalophilic bacillus. Biochem Int. 1983 Nov;7(5):577–583. [PubMed] [Google Scholar]

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