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. 1988 Dec;54(12):3169–3172. doi: 10.1128/aem.54.12.3169-3172.1988

Tn5-Mediated Cloning of a Genetic Region from Pseudomonas putida Involved in the Stimulation of Plant Root Elongation

Ran Lifshitz 1, Helene Guilmette 1, Maya Kozlowski 1,*
PMCID: PMC204445  PMID: 16347807

Abstract

Transposon (Tn5) mutagenesis was applied to Pseudomonas putida GR12-2R3, which promotes root elongation (a phenotype designated Pre) of Brassica campestris under gnotobiotic conditions. Of 3,000 Tn5 transconjugants, only one mutant that lost Pre activity but remained prototrophic and capable of plant root colonization was detected. This mutant was complemented by plasmid pRE53, which contained a 15.0-kilobase DNA insert isolated from a parental strain. The complemented mutant regained full Pre activity comparable to that of the wild type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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