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. 1988 Dec;54(12):3173–3176. doi: 10.1128/aem.54.12.3173-3176.1988

Transfer and Expression of Lithoautotrophy and Denitrification in a Host Lacking These Metabolic Activities

Beate Schneider 1, Anke Nies 1, Bärbel Friedrich 1,*
PMCID: PMC204446  PMID: 16347808

Abstract

The conjugative 450-kilobase-pair megaplasmid pHG1 from Alcaligenes eutrophus H16 was transferred to the herbicide-degrading soil bacterium A. eutrophus JMP134. This transfer was achieved by means of RP4 mobilization and a Tn5-Mob insertion provided in trans on the megaplasmid replicon. Although kanamycin-resistant transconjugants also occurred with other gram-negative species such as Rhizobium, Agrobacterium, and thiobacteria, A. eutrophus JMP134 was the only recipient which stably maintained the megaplasmid. pHG1-containing transconjugants derived from JMP134 expressed all metabolic functions associated with the plasmid: the ability to oxidize hydrogen through catalysis of two hydrogenases, to assimilate carbon dioxide via the Calvin cycle pathway, and to grow with nitrate anaerobically. All of these metabolic activities were absent in the original strain JMP134.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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