Abstract
1 Human erythrocytes suspended in isotonic saline haemolyse in the presence of both Ag+ ions and sulphadimidine. 2 Neither Ag+ ions nor sulphadimidine on their own will haemolyse erythrocytes suspended in isotonic saline. 3 At constant Ag+ ion concentration the degree of haemolysis of saline-suspended erythrocytes depends upon the concentration of sulphadimidine. 4 Human erythrocytes suspended in isotonic sucrose (chloride-free) haemolyse in the presence of Ag+ ions. 5 Sulphadimidine in chloride-free sucrose competes with erythrocytes for Ag+ ions resulting in stoichiometric protection of the erythrocytes from the haemolytic action of Ag+ ions. 6 Haemolysis occurs when each erythrocyte receives approximately 1.2 X 10(9) Ag+ ions whether suspended in saline or sucrose. 7 Sulphadimidine acts as a carrier for Ag+ ions and so prevents their precipitation as AgCl when erythrocytes are suspended in saline.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker P. F., Willis J. S. Binding of the cardiac glycoside ouabain to intact cells. J Physiol. 1972 Jul;224(2):441–462. doi: 10.1113/jphysiol.1972.sp009904. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ballinger P. M., Griffin M. M., Itzhaki S., Stevens F. S. Evidence for the carriage of silver by sulphadimidine: inhibition of proteolytic enzymes. Br J Pharmacol. 1982 Sep;77(1):147–151. doi: 10.1111/j.1476-5381.1982.tb09280.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox C. L., Jr Silver sulfadiazine--a new topical therapy for Pseudomonas in burns. Therapy of Pseudomonas infection in burns. Arch Surg. 1968 Feb;96(2):184–188. doi: 10.1001/archsurg.1968.01330200022004. [DOI] [PubMed] [Google Scholar]
- Joiner C. H., Lauf P. K. The correlation between ouabain binding and potassium pump inhibition in human and sheep erythrocytes. J Physiol. 1978 Oct;283:155–175. doi: 10.1113/jphysiol.1978.sp012494. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDougall I. A. Use of silver sulphadiazine in the treatment of burns at Royal Perth Hospital. Med J Aust. 1972 May 6;1(19):979–981. doi: 10.5694/j.1326-5377.1972.tb108037.x. [DOI] [PubMed] [Google Scholar]